Embryo Transfer in Cattle Part 3 | Sam Houston State University - Edubirdie

University:
Sam Houston State University
Course:
BIOL 1406 | General Biology I
Academic year:

2019
Views:

152

Pages:

10
Author:

5CelestialChronicle

The Flush  Once the donor cow has been bred, the embryos are allowed to grow for six days.  During this time the embryos also travels down the reproductive tract from the oviduct (the site of fertilization) to the uterus where they can be flushed out.  On the seventh day, the embryos are ready to be removed. This process is called flushing. The Flush (continued)  Embryo professionals use a non-surgical method to remove the embryos. The process requires experience and a patient, steady hand. The Flush (continued)  An injection of lidocaine is given prior to the flush to reduce pressure and stress on the donor cow and to make the flush easier for the ET professional. The Flush (continued)  To begin the flush, a catheter is passed through the cervix into one uterine horn. The Flush (continued)  The catheter contains a balloon that is inflated with a saline solution in order to seal the entrance to the uterus so fluid and embryos are not lost. Removing the Embryos  The uterine horn is filled with flush media and massaged to allow the embryos to flow out of the tract.  This process is repeated several times in each uterine horn. Collecting the Embryos  Embryos are carried out of the reproductive tract through plastic tubes and collected in a filter with the flush media.  The pores in the filter are smaller than the embryos so excess fluid drains out of the filter without losing the embryos. Injecting Penicillin  After the embryos have been flushed out, uterus injected with penicillin to kill any missed embryos or infections. Embryo Statistics  An average of 7-10 embryos is collected from each flush.  However, the number of embryos obtained from a single flush may range anywhere from 0-60. Separating the Embryos  In the lab, embryos are separated from the flush media and examined under a microscope to determine their quality and stage of development.

Related Documents

Embryo Transfer in Cattle Part 3

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