University:
Texas A&M University
Course:
BIOL 213 | Molecular Cell Biology
Academic year:

2023
Views:

35

Pages:

18
Author:

behend6abs

FUNDAMENTAL TISSUES AND MITOSIS Part III Images of mitosis Objectives • Operate the “virtual microscope” • Describe steps for tissue processing and discuss different types of microscopy • Interpret cytological significance of differential staining by hematoxylin and eosin (H&E) • Recognize and identify cells in microscopic tissue preparations • Understand that morphology reflects the function of cells Part III • Identify stages of mitosis and cellular structures associated with mitosis Mitosis • Mitosis is cellular multiplication • This is the means by which organisms grow • Cancer is essentially uncontrolled mitosis • We will look at onion root tip and fish egg cells to identify the stages of mitosis Onion root tip Interphase Interphase nucleus Onion root tip Prophase Onion root tip Metaphase Spindle fibers Onion root tip Anaphase Centrosomal area Onion root tip Telophase Fish egg mitosis Interphase Interphase nucleus Fish egg mitosis Prophase Fish egg mitosis Metaphase Spindle fibers Fish egg mitosis Anaphase Centrosomal area Fish egg mitosis Telophase EM 25 & 26 Describe the composition of the nucleolus and the nucleus. What happens to the nucleolus and nuclear membrane (envelope) during mitosis? EM 31 • What is the function of centrioles? Clinical Correlation • Malignant vs. benign tumors • Increased mitotic figures and abnormal mitoses helps distinguish • Anti-mitotic drugs are used in cancer chemotherapy • Ex: Taxol • These drugs also effect normal cells with high proliferation rates causing undesirable consequences Study Questions 1. Does a highly active cell contain a euchromatic or heterochromatic nucleus? 2. Would the cytoplasm of a cell that contains large quantities of protein stain eosinophilic or basophilic? 3. Is a rounded (spherical) nucleus indicative of a squamous, cuboidal or columnar cell? 4. Does hematoxylin preferentially stain chromatin or collagen? (2-3 sentences) 5. Are polysaccharides and mucopolysaccharides best stained with eosin, Sudan black, hematoxylin, or periodic acid – Schiff? 6. At which stage of mitosis do sister chromatids separate from each other? 7. During which phase of interphase does the first “checkpoint” occur? What is the significance of the p53 protein? From: Douglas P. Dohrman and TAMHSC Faculty 2012 Structure and Function of Human Organ Systems, Histology Laboratory Manual Use your atlas! Use your atlas! pancreas blood stomach Atlas of Normal Histology by DiFiore testis