Cellular Intoradient-clathrin coated Pits, liped rafts, endocytosis and wous uncoating mechanisms Cellular Interactions: refers to the dissect interactions between cell surfaces that play a coucial ocole in the development & function of multicellular organisms. → these unteractions allow cells to communicate with sach other in sesponse to changes in their micro Enuironment. Clathrin Coated pits: are specialized patches at the plasma membrane that concentrate sreceptors, curve to form an invagination & bud off with their receptor cargo in the process of clath- mediated endocytosis, Clathin coated pits, fare cellular component coated pils all regions of the cell membrane Their cytoplasmic surface is ike structure made Coated with bristle of clathrin inside clathrin coated pits traffiking Receptor of specific Proteins are mainly used four Substance into cell. AR2 Adaptin clathrin Plasma uncoating →clathrin mediated endocytosis (COP) is use to absarb metabolites I harmones other proteins as dect Some times wruses also Enter into the cell by invagination of plasma membiare → Cep all mainly used to transport specific substances into the cell by following steps. SCP-I The ligand or molecule Erotein) attach to the specific receptor Step-Ⅱ the suceptar complex bind to Ike adaptar Stop- now these complex fier, receptor, Putn 24 adaptor attached to the clathrin to farm vesicle ar clathrin Coated Fits Step-IV After formation of cop, this cap لاش aulased from plasma membrane & intro- duced into the cell Step- Dater this vesicle enders unto Early Endasomel ande passes thrayh lysosome liers the activated receptar transport thrayh Early endosomes then lysosomer for degra- dation) function of the function of clathrin coated CCP Pits clathrin endarytoju used for specific uptance of certain substance required by cell → It is also used in gregulation of trans mem- -brane Single thansduction Lipid Rafts is Part of cell membrane (mare importantly sepciallized part of cell membrane) Definition the plasma membrane / cell membrane of cell containing combination of Glycosphino golipids and protein secaptors orgenied in glyco proteins are called liped, jarls con Liped aft liped saft PER afperana! "Lipid safe or Rotein cholesterol glyce sphingolipid omains are slightly thicker than the other fall of the membrane Composition: The lipid crafts contain high level of Sphingolipidi and cholesterol[3-5] foldes in bilayer → cholesterol acts lice dynamic glue cotich holds lipid rafts together found! lipid orafts are mare common in plasma membrane. They also present in golgi apparatus and lysosomes Identification lipid rafts are identified in. membrane by Exposure to detergent like triton x-100 (membrere) → Generally other things rewhen exposed / added this triton x-100, membrane will dissolved. But, if membrane possess / contain lipid raft it shows resistant to triton x-100 detergent P Role/Imp of lipid grafts! lipid safts are mainly acts as signaling molecule and also use for cleaning membrane fluidity and membrane protein traffiking Types of Liped safts! lipid safty all of 2 types Manar liped, raft 4) Caveolin tiped saft [glotin 1) Planar lipid raft! It is mostly found in neurons and Protein. are enriched in Flotillin ii) caveolin lipid saft Eglutin) small flask shaped invagination of plasma membrare Enriched in Cavesten protein. * Endocytosis and uncoating mechanism!- Endocytosis! Transpotation of the molecule inside the all. → In Enveloped wuses they farm Bud formatio lenown as Budding In non Enveloped viruses directly enters into cell by endocytosis machanism In endocytatis mechanism different types of methods are their They ale! 1) Clathalin mediated endocytaty ⅱ) Caveolin mediated Endocytaju ⅲ) macropinocytosis w) clathrin-caveolin mediated Endocytosil 1) clatharin mediated endaytosis! → It is the most common mechanism for Small and medium size wowser → It is also called as receptar mediated endo. eytosis because the molecule attached to the seeceptar on the plasma membrane for transporting into the Coll →In this endocytosis, they form clathalin Coated pits ar vesicals to thansport the molecules ⅱ) caveolin mediated endocytosis! →The endocytic orole of caveolin in cell life is poorly defined Hel the transportation of molecule is carried by the activated ligand → the caveoler vesical formation is used to pansport the vitus unto the cell. ⅲ) macropinocytosis! → this endocytosis mechanism is used Cellular factors / materials non, Latra → It is mainly involves in transpotation of saternal factors, like growth factars The key event in micropinocytosis is they contain activated receptar for transpotation 29!- receptar tyrosin, untegrine → By this mechanism, the unfectious wrises of dyfuent family including large wrusel such as pox uris, Paramita viius are transpoted w) clatkarin-caveolin mediated endocytosi!- It is found in members of polyoma winser these alu with non-enveloped wuses repliates in the host all mainly they transport lipids & other specific receptors This endocytosis uses vesical formation to hansport the wries inside the cell. → the virus containing vesical farm are delivered to sarly endosomes via cate endo Somel to Endoplasmic reticulum.