7/1/2023 VIRAL METADATA RESOURCES [VMR] vme: VME stande for viral metadata resources →This VMe provides list of all the viruses and Viruses species through downloadable Spreadsheete we can find all well characterized Via uses including gene bank accession number for genomic sequencing and we can also find out the virus name, attenuation geriomic Composition and host sour Viral melagenomics - virome Metagenomics:- →The study of the structure and function of entire nucleotide sequence isolated and analysed fram alu organisms [typically microbes] directly from Environment on host in bulk Sample. → whereas genomics refers to study of single organism genomel genome of single organtim and metagenomics refers to study of different organisms genomes Viral Melagenomics: → study of viral genetic material obtain from Enviromental DNA Sample (ie, natural source, soil) os Clinical DNA sample obtain from host. →This viral metagenomics method can be applied to study viruses in any system and has been used to describe various Viruses associated with Cancer tumours, extreme environment, terrestrial ecosystem, blood & faces of human. Visome Visome refers to the assemblage of visvece that is often Investigated and described by metagenomic Sequencing of Visal nucleic acids with particulas ecosystem organismos holuberon't viral metagenomics is a Culture Independent methodology that provides Insights on viral atrastly, abundance and functional potential of viruses within the environment How Analyze I study the genome/metagenomic process. Sampling from habitat - Extraction of genetic material from Samples like Erivisonment (eg: Soil, sea water) on from human gut filtering the particles, typically by size -Separation of organisms from the given sample based on Size. Lysis and DNA Extraction - Breakdown/lysis of an organiem In order to Isolate (sepurate) genetic material Cie, DNA & RNA) Cloning and library (0) Trotated genetic making allow Construction for multiplication by cloning technique Cie, making of identical Copte). Os prepating the newly formed genetic maternal Ints a form that 14 compatible with the Seqventing Sequenting the Clones: Arranging the genetic maternal 1.e.. determining the exact sequen of nucleotide on bases BA DNA Sequence assembly into contigs and Scaffords The arranged nucleotide sequence inserted into contige and scafford's regions of Chromosome.