University:
The University of Tampa
Course:
BIO 198 | General Biology I
Academic year:

2023
Views:

228

Pages:

3
Author:

Angel Huynh

Gel Electrophoresis Virtual Lab 1. What is Gel Electrophoresis used for? Gel Electrophoresis is whenever scientists need to sort DNA strands according to their length. This technique is also used to separate other molecules, like proteins. 2. List the steps in Gel Electrophoresis. a. Make the gel b. Set up the gel apparatus c. Load the DNA sample into the gel d. Hook up the electrical current and run the gel e. Stain the gel and analyze the results 3. What is the purpose of running DNA Standards along with the DNA sample? The purpose of running DNA standards along with the DNA sample is that running it on the gel will give you a reference by which to estimate the lengths of DNA strands in your sample. 4. In which direction does the DNA run on the gel – negative to positive or positive to negative? Repelled by the negative end, the DNA moves through the gel toward the positive charge at the other end. 5. Read the following gel. Put the estimated base pair lengths in the boxes. Estimated BP: 6,000 2,000 1,000 6. Which fragments of DNA moved the farthest through the gel – large pieces or small pieces? The smaller pieces moved the farthest through the gel. 7. Gel Electrophoresis is often used in forensics. Examine the gel below. Based on the evidence, which individual matched the DNA evidence left at the crime scene? How do you know? Based on evidence, suspect #2 matches the evidence. I know this because the gel shows the same exact marks as the evidence DNA found. 8. What could be a possible source of error when performing or analyzing Gel Electrophoresis? One possible source of error when performing or analyzing Gel Electrophoresis could be if too much or too little DNA was loaded into a well. Another possible source of error could be contamination of the DNA sample. 9. List one specific reason why we would run a gel electrophoresis on someone and explain your answer. One specific reason why we would run a gel electrophoresis on someone is in forensics. It is typically used to acquire a DNA sample of a suspect. It helps to see if sample DNA matches the suspects DNA. Another example is in genetics. It is used to display a clear picture of DNA.

Gel Electrophoresis Virtual Lab Answer Key

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