TESTING THE EFFECTS OF SEDATIVE DRUGS Practical Objectives Students can get to know, learn andnervous system. practice the influence of suppressive drugs Basic theory Sedative hypnotic drugs have pharmacological effects similar to general anesthetics,if these drugs are given in larger doses, the effect is the same asgeneral anesthetic. Both types of drugs have similar mechanisms of action in suppressingcentral nervous system.Sedative drugs (antipsychotics) differ in effect from hypnotics because they do notcauses an anesthetic effect. Examples: Chlorpromazine and reserpine, action in emphasis on The central nervous system is not so strong/deep that it only causes a sedation effect. Effect sedatives can affect the motor coordination abilities of test animals. So the sedative effect Testing will be carried out in this experiment with test animals using parameters rotarod, gripping power, corneal reflex, and pupil dilation/diameter. Chlorpheniramine is an antihistamine preparation but has sedative side effects similar to drugs sedative. This antihistamine property has nothing to do with its ability to function inhibits/antagonizes histamine. Tools and materials Tool a. Rotarod (rotating rod) and syringe b. Rat container c. Gloves d. Marker (to mark mice) e. Stopwatch Material:Mice (test animals), diazepam (sedative drug) Test Animals:Mice: 10 mice Work procedures a. Mice were divided into 2 groups, 1 treatment group (@5 mice) and 1 control group (@5 mice). Before treatment with the test animal drug placed on the rotarod for 5 minutes for adaptation. b. the control group was injected with aquabidest with a volume equivalent to IM diazepam and the treatment group was injected with a dose of diazepam conversion but humans to mice (5 mg/mL diazepam preparation for 50 kgBW) c. At 15, 60, and 120 minutes, the mice were placed on the rotary rod for 2 minutes. d. Note the number of times the test animal falls off the rotarod e. During the experiment observe: • Body and corneal reflexes • Grip force on wire mesh • Changes in pupil diameter f. Tasks for each practitioner: Analyze qualitative and quantitative data by comparing group effectstreatment and control. g. Question 1. Why do mice need to be adapted before the experiment? 2. Indications include loss of reflexes of the body and cornea, powergrip, and pupil changes? 3. Explain how diazepam has a sedative effect?