BILE EXPERIMENT 1. Properties of Bile Note the color, odor, consistency, pH and specific gravity of the fresh bile. 2. Mucin and Inorganic Substances Acidify 25 mL of diluted bile with 10% acetic acid. Musin will settles. Strain and check the filtrate for: a. Chloride Take some of the filtrate and acidify it with nitric acid, then add silver nitrate. A white precipitate (AgCl) indicates its presence chloride. b. Sulfate Acidify the filtrate with dilute HCl and add 2% BaCl2. White precipitate indicates the presence of sulfate. c. Phosphate Add 1 mL of filtrate with 10% urea and 10 mL of reagent special molybdate. Mix, then add 1 mL of special FeSO4 solution. The blue color that becomes more pronounced after being left alone indicates its existence orthophosphate. Note: After addition of special molybdate, the solution must be acidic. 3. Bile Pigments Bile pigments mostly come from the breakdown of blood cells red. The most abundant pigments are bilirubin and biliverdin. Bilirubin red-yellow-brown in color, while biliverdin is green. All This pigment is responsible for the golden yellow color of bile. Oxidation of this pigment produces other pigments of various colors. a. Gmelin Test Put 3 mL of concentrated nitric acid into a test tube. Carefully add 3 mL of diluted bile so that both not mixed. Note the color visible on the second border fluid. Check the sensitivity of this test by using more bile runny. b. Smith test Put in a little very diluted bile and a few drops 0.5% iodine (in alcohol) into a test tube so two liquid layers are formed. Note the dark green or ring greenish blue under the iodine layer. 4. Bile Acid Human bile contains 4 types of bile acids, namely cholic acid, chenodioxycholic acid, dioxylic acid, and lithocholic acid. Cholic acid and Cenodioxycholate is the main bile acid. Normally bile acids formed at relatively low speeds. Petternkofer test Add 5 drops of 5% sucrose to 5 mL of diluted bile. Add 3 mL of concentrated H2SO4 carefully through the tube wall so that 2 layers of liquid are formed. What is visible? Compare this test with the test Molisch.