Reagents for Analgesic

Reagents for analgesic compounds, test tubes, dropper pipettes, tube racks, glass objects, cover glasses, microscopes, spirit lamps, sublimation rings Work procedures 1. Aminophylline Build Formula a. Substance when burned → smell of pandan b. Fluorescence: weak blue (in water/H2SO4runny) c. Substance + Cu Acetate → purple + K2Hg4→ ↓ white d. Substance + HCl → ↓ theophylline e. Substance + Aqua Bromine → ↓ white crystals f. Substance + Nessler → ↓ white g. Zat + Mayer → ↓ purple h. Substance + Cu. Acetate → ↓ purple i. Murexide reaction: positive j. Parri's reaction: negative k.Crystal reactions with Dragendorf, Fe Complex. 2. Theophylline Build Formula a. Millon's reaction → positive b. Parri's reaction → positive c. Roux reaction → stable green d. Substance + Water + NaOH 5 drops heat + AgNO3→ clear gel cannot be poured e. Substance + aqua Bromine → ↓ white f. Substance + 1 drop of HCl + 1 drop of H2O2, heat over WB, remaining brown + 1 drop of NH4OH → red purple g. 10mg sulfanilic acid + 5 drops of dissolved HCl chilled on ice. Into the solution, mix 10 mg of the substance in 1 ml of heated NaOH, cool it on ice → red h. 10 mg substance + 1 ml HCl + 10 mg KClO4, evaporate until dry → remaining red brown + 1 NH4OH → red violet (= Murexide) i.Crystal Reaction : - Substance + 1 ml diluted HCl + HgCl2→ crystal - Substance + 1 ml HCl diluted + Dragendorf heat briefly → crystals - Substance + NH4OH + H2SO4→ rosette crystal 3. Coffee Build Formula a. Murexide reaction: Substance + 1 drop of H2O23% or KClO3solid + 1 drop of heated 25% HCl → slightly orange + NH4OH → purple b. Aqueous solution of substance + I2does not occur ↓ + HCl → ↓ brown, dissolves in excess NaOH c. Parri's reaction → positive d. Substance + Cu acetate powder + 1 drop of water → violet color e. Substance + 1 tts H2O2(at KClO4solid) + 1 tts 25% HCl, heat over water bath → yellow + NH3 → Purple f. Francois reaction → blue g. Substance + Water + NaOH 5 drops heat + AgNO3→ ↓ black h. Lar. Saturated substance + HgCl25% → ↓ white, heat → ↓ needle crystal. i. Zwikker reaction: (1 ml pyridine 10 %+ sol. CuSO4). Substance + reagent → colorless long rod crystals (microscope) j.Crystal reactions with Dragendorf, HgCl2 4. Isoniazid Build Formula a. Substance + AgNO3,heat → reduce b. Substance + AgNO3ammoniacal → reducing c. Substance + Fehling → reducing d. Zat + Luff → reducing e. Substance + Vanillin + methanol + HCl → yellow green f. Substance + KMnO4→ neutral slow colors, - Acid (+H2SO4) : (-) - Base (+NaOH): fast green g. Substance + phosphomolybdic acid + NH4OH → ↓ blue h. Astringent + Roux → red brown i. Substance + DAB HCl → orange yellow j. Substance + NaOH heats out NH3 k. Substance in methanol + HCl + DAB → red brown sometimes yellow. l.Crystal reactions with Dragendorf, HgCl2, Fe. Complex, Picric Acid. 5. Acetosal Build Formula a. Substance + FeCl3→ purple. b. Zat + Marquis → blood red c. Zat + Frohde → instant purple d. The substances in alcohol + zwikker → ↓ are very subtle e. Substance + H2O + CaCO3→ Shake, strain. Filtrate + FeCl3→↓ Light brown f. Sublimation: look at the crystals under a microscope 6. Antalgin Build Formula a. Substance + Mayer → positive (Yellow white precipitate) b. Substance + Bouchardat → positive c. Substance + HNO3→ blue, green yellow d. Substance + FeCl3→ blue, green yellow e. Substance + HCl + NaOCl → blue, green f. Substance + dilute HCl + FeCl3→ blue → still → red → colorless g. The substance is reduced with KMnO4→ color is lost h. Diazotation: HCl + NaNO2+ beta Naphthol → ↓ orange turns brown turns green i. Substance + AgNO3 metallic) j. Crystal Reaction: Complex Fe, K4Fe(CN)6 → crystals are formed (purple with silver deposits 7. Acetaminophen Build Formula a. Substance + water + FeCl3→ purple blue b. Boil substance + HCl + cool water → no precipitate is formed c. Substance + p-DAB HCl, a yellow precipitate is formed. d. Substance + 3 N HCl, heat for 5 minutes → the diazo reaction produces an orange red color e. Substance + Diazo A and B, an orange solution is formed. f. Substance + NaOH + ethanol heated → solution smells of isonitrile (foul smell) g. Fluorescence test: blue green 8. Papaverine Build Formula a. Zat + Erdmann → purple, green blue in cold conditions. b. Zat + Marquis → Brown c. Zat + Frohde → Violet red to Brown d. Substance + H2SO4(p) → purple sometimes green blue e. Substance + 5 ml H2SO4dilute → heat → + 1-2 drops of FeCl3→ violet color → after cooling + 1 drop of concentrated nitric acid → red color f. Bosmann: lar substance + H2SO4(e) + KMnO4, shake with CHCl3→ CHCl solution 3Violet g. Coralyn reaction: 10 mg of test substance + 1 ml of acetic anhydride + 3 drops of concentrated sulfuric acid → greenish yellow fluorescence h. Substance + 5 ml of water containing 0.5 ml of dilute HCl + 5 drops of potassium ferricyanide → orange yellow precipitate → observe the crystals under a microscope (differentiate from other opium alkaloids) 9. Ephedrine Build Formula a. Mayer: negative b. Bouchardat: positive c. Iodoform reaction: positive d. Substance + H2SO4(e) + NaCl → 6 drops of 0.1 N NaOH, heat on wb → red, after cold → violet. e. Substance + heated NaOH + Aqua Iod → Iodoform f. Chen and Kao reaction: Substance + 1 ml of water + 1 drop of CuSO salt4+ 1 ml NaOH 4N → Violet, shake with ether → red/purple red g. Substance + CuSO4dilute + NaOH → purple h. Substance + sulfanilic acid + NaNO2→ dark red/orange i. Substance + 2 ml water + a few drops of NaOH + potassium ferric cyanate → heat → aldehyde smell j.Crystal reactions with Dragendorf, solid K-oxalate.