Determination of Calcium Levels - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

362

Pages:

3
Author:

Ethan V.

MATERIAL VI DETERMINATION OF CALCIUM LEVELS (QUANTITATIVE) Basic theory Canned fish products that include bones (salmon or sardines) also contain a lot of calcium, but sliced fresh fish without bones is not a high source of calcium. 1) Deficiency and Excess of Calcium The negative impacts of mineral deficiency are often not visible until they reach adulthood. Calcium deficiency during adolescence is a cause of osteoporosis in old age. Calcium deficiency can cause: Dental caries (tooth decay). 2) Bone growth is imperfect. 3) It is difficult for blood clots to occur. 4) Muscle spasms occur. Apart from the calcium deficiency above, an imbalance of calcium and phosphorus can also cause harm. Because phosphorus can increase parathyroid hormone. If this imbalance is not addressed, calcium deficiency continues while phosphorus buildup continues Method Principles Arsenazo III is chemically stable and has a very high affinity for calcium in the neutral pH range. In this test system, Arsenazo III forms a blue complex of Arsenazo III calcium with an absorbance maximum at 650 nm. The calcium concentration is comparable to the absorbance of the blue colored Arsenazo III-calcium complex. Procedure Wavelength : Hg 623-650nm ( 630-670 nm ) Optical path : 1 cm Incubation temperature : 20 – 25°C or 37° Material : Blood Sample 10-15 ml Reactor - Phosphate Buffer, pH 7.5 - 8-Hydroxyquinoline-5-sulfonic acid - Arsenazo III Detergents Specimen (biological sample) Serum, plasma heparin or plasma EDTA Stability: • 10 weeks at a temperature of 20-250C, • 5 months at temperature 4-80C and • 3 months months at a temperature of -200C Tool : 1. Reaction tube 2. EDTA 3. Centrifuge 4. Kits 5. Spectrophotometer 6. Myropipette 7. Implementation Pipette into test tubes Blank Std./Cal Sample Reagent 1000 µl 1000 1000 Sample - - 10 Std./Cal - 10 - Water 10 - - Mix, incubate for 5 min. At 20-25°C/37°C. Measure absorbance of std./cal. and sample against reagent blank. The color is stable for at least 1 hour.

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