Urobilinogen Urine Chemical Examination - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

353

Pages:

2
Author:

Ethan V.

PRACTICUM II URINE CHEMICAL EXAMINATION UROBILINOGEN 2.5 Urobilinogen Practicum : Determination of Urobilinogen Objective : To detect liver problems Urobilinogen is a substance resulting from the breakdown of bilirubin in the body. Bilirubin itself is a yellow substance found in the liver and functions to break down red blood cells. MMost of the urobilinogen leaves the body in the feces, but a small part is absorbed by the ,blood and returned to the liver. From the liver, urobilinogen exits again through the bile with a small portion entering the kidneys and excreted from the body with urine. 2.5.1 Wallace diamond method Working Principle: Reaction of urobilinogen in urine with p-dimthyl amino benzaldehyde In acidic conditions it will form a complex compound which is wine red in color. Tools and materials 1. Test tube + Rack 2. Tube 3. Pipette scale 4. Balm 5. Wallace diamond reagent (Erlich reagent) consisting of p-dimthyl amino benzaldehyde 2g 6. 150 ml concentrated HCl and 300 mL distilled water Work procedures : 1. Pour 10 ml of urine into a tube, add 1 ml of Wallace diamond reagent 2. Mix and leave for 3-5 minutes (don't exceed 5 minutes) 3. The results of the examination are determined as follows: Look from top to bottom at the bottom of the test tube which is set up perpendicular to a piece of white paper as the base, if there is a red color that occurs: - If only a faint red color is visible then the experiment is considered complete - If the red color is very clear, continue the examination with urine dilution as follows: * make a series of urine dilutions from 10x-100x or if necessary even higher 4. Using the diluted urine, do (repeat) the examination according to the Wallace diamond as in steps 1-3 5. The results of the examination are reported by stating the highest dilution that still shows a red color (+) and also mentioning the dilution that does not produce a red color (-) Example: Dilution 1:40 (+) Dilution 1:30 (-) 6. The test is considered normal if the results are 1:20 positive and 1:30 negative Interpretation of results: Negative (-) : at 40x dilution Positive (+) : at 20x dilution

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