God's Glucose Examination | Buffalo State University - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

390

Pages:

2
Author:

Ethan V.

MODULE 2 GOD'S GLUCOSE EXAMINATION - PAP OBJECTIVE 1. General Objectives Students are able to understand the principles of glucose examination on serum samples. 2. Specific Objectives a. Students are able to carry out glucose examinations on serum samples. b. Students are able to interpret the results of glucose examinations on serum samples. METHOD “GOD-PAP” Photometry: Enzymatic Test PRINCIPLE Determination of glucose after enzymatic oxidation by glucose oxidase. The colorimetric indicator used is quinoneimine, which is produced from 4-aminoantipyrine and phenol by hydrogen peroxide under the action of a peroxidase catalyst. TOOLS AND MATERIALS Tool:  Micropipette + tip  Spectrophotometer  Serology tube  Tube rack  Beaker glass Material: • Reagent Phosphate Buffers pH 7.5 250 mmol/L Phenol 5 mmol/L 4-aminoantipyrine 0.5 mmol/L Glucose Oxidase (GOD) ≥ 10 kU/L Peroxidase (POD) ≥ 1 kU/L WAYS OF WORKING Blank Sample Sample - 10 μL Blank 10 μL 1000 μL Reagents 1000 μL 1000 μL 10 μL Mix, incubate 20 minutes at 20⁰C-25⁰C or 10 minutes at 37⁰C. Read the Blank Absorbance within 60 minutes INTERPRETATION OF RESULTS mg/dl mmol/L Newborn : mg/dl mmol/L Fetus 53-158 3.5-8.8 1 hour 36-99 2.0-5.5 2 hours 36-89 2.2-4.9 5-14 hours 34-77 1.9-4.3 10-28 hours 46-81 26.45 44-52 hours 48-79 2.7-4.4 1-6 years 74-127 4.1-7.0 7-19 years old 70-106 3.9-5.9 Adult (Plasma) 70-115 3.9-6.4 Children

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