Albumin Examination Method | Buffalo State University - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

313

Pages:

2
Author:

Ethan V.

ALBUMIN EXAMINATION METHOD The method used is BCG (Bromocresol green) PRINCIPLE In the presence of BCG (Bromocresol green) at a slightly acidic pH, serum albumin produces a color change from the yellow-green indicator to green-blue whose absorbance is measured on a spectrophotometer with a wavelength of 632 nm. TOOLS AND MATERIALS Tool : - Serology tube - Cuvette - Spectrophotometer - Measuring pipette - Ball pipette - Micropipette - Tip - Serology tube rack Material: - Albumin reagent - Serum control - Serum samples - Aquadest WAYS OF WORKING 1. All tools and materials are prepared first. 2. Reagents and samples are conditioned at room temperature. 3. Prepare 3 serology tubes and label them as blank, standard and test. 4. Pipette 2 mL of albumin reagent and put it in the three tubes. 5. Add 0.01 mL of distilled water to the blank tube. 6. Add 0.01 mL of standard serum to the standard tube. 7. Add 0.01 m L of serum sample to the sample tube. 8. Each of them was put into a cuvette and the absorbance was measured with a spectrophotometer at a wavelength of 632 nm. NORMAL VALUE 3 –4.5 g % albumin

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