Module 7: Creatinine Examination | Buffalo State University - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

179

Pages:

2
Author:

Ethan V.

MODULE 7 CREATININE EXAMINATION METHOD The method used is methodJaffe Reaction PRINCIPLE Creatinine will react with picric acid in an alkaline atmosphere to form a complex compound which is orange yellow in color. The intensity of the color formed is equivalent to the creatinine level in the sample, which is measured with a photometer with a wavelength of 490 nm. TOOLS AND MATERIALS Tool : − Spectrophotometer − Test tubes and tube racks − Tip − Micropipette 10 ul and 1000 ul − Beaker glass Material : − Tissue − Serum Samples − Creatinine Reagent: R1: Sodium hydroxide 0.2 mol/L R2: Picric acid 20 mmol/L − Creatinine standard is 2 mg/dL − Aquades WAYS OF WORKING 1. Prepare the tools and materials used and condition them at room temperature. 2. Prepare 3 test tubes that have been labeled blank, standard, test. 3. Pipette each into a tube: Blank Standard Sample Aquadest 25µl - - Standard - 25 µl - Sample - - 25 µl Monoreagent 500 j 500µl 500µl 4. The mixture was homogenized, then incubated for 1 minute. 5. Then the absorbance of the solution was read with a spectrophotometer at a wavelength of 490 nm. 6. Absorbance is recorded, then the creatinine level in the sample is calculated. INTERPRETATION OF RESULTS Normal creatinine values in the Jaffe reaction method are: Man: 0.6 - 1.1 mg/dL Woman: 0.5 - 1.9 mg/dL

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