Module 4: SGOT Examination - Edubirdie

University:
Buffalo State University
Course:
CHE 111 | Fundamentals of Chemistry
Academic year:

2024
Views:

332

Pages:

3
Author:

Ethan V.

MODULE 4 SGOT EXAMINATION (SERUM GLUTAMIC–OXALOACETIC TRANSAMINASE) METHOD The method used is the UV spectrophotometric method based on IFCC (International Federation of Clinical Chemistry and Laboratory Medicine) (Modification) PRINCIPLE Aspartate amino transperase (ASAT/AST) catalyzes the transaminase of Laspartate and 2-oxogluttarate to form L-glutamate and oxaloacetate> Oxaloacetate is reduced to L-milate by the enzyme malate dehydrogenase (MDH) and nicomamide Adenine denodeotide 9NADH) is oxidized to NAD. The amount of oxidized NADH is directly proportional to AST activity and is measured photometrically on a spectrophotometer with a wavelength of 340 nm. TOOLS AND MATERIALS Tool: - 100 µl micropipette - 500 µl micropipette - Blue tip - Test tube - Cuvette - Spectrophotometry Material: - ERBA ASAT (SGOT) reagent - R1 R2 Tris buffer (pH 7.8) 110 mmol/L L-Aspartate 340 mmol/L LDH > 4000 U/L MDH > 750 U/L CAPSO 20 mmol/L 2-Oxoglutarate 85 mmol/L NADH 1.05 mmol/L - Serum samples - Standard WAYS OF WORKING 1. Prepare all the tools and materials that will be needed 2. Monoreagent is made by mixing 4 parts of R1 with 1 part of R2, then waiting 30 minutes. 3. A total of 500 µl of ASAT monoreagent (GOT) is put into a reaction tube 4. Add 50 µl of serum sample into a test tube containing the reagent. 5. The absorbance of the solution was measured using a spectrophotometer at a wavelength of 340 nm. 6. Absorbance is read after 1 minute. The absorbance was read again after the next 1.2 minutes. 7. The results of the sample absorbance data are recorded and then the SGOT levels are calculated from the serum samples examined. INTERPRETATION OF RESULTS 1. By activation of pyridoxal - S- phosphate a. Adult female : < 31 U/L b. Adult male : < 35 U/L c. Children 1- 3 years : < 50 U/L 4- 6 years: < 45 U/L 7- 9 years: < 40 U/L 10 –12 years: < 40 U/L 13 –15 years: < 35 U/L 16 –18 years old: < 35 U/L 2. SGOT without pyridoxal –S –phosphate activation a. Adult female: < 31 U/L b. Adult male: < 35 U/L

Related Documents

Module 4: SGOT Examination

Module 4: SGOT Examination - Page 1

Module 4: SGOT Examination - Page 2

Module 4: SGOT Examination - Page 3

of 3

Recommended Documents

Antibiotics Laboratory: Chemical Reactions and Color-Based Identification of Antibiotics

Antibiotics Laboratory: Chemical Reactions and Color-Based Identification of Antibiotics

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lab

Classification Reactions - Lecture Note

Classification Reactions - Lecture Note

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Redox Titrationis Study Materials

Redox Titrationis Study Materials

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Nitritometry Titration Lecture Notes

Nitritometry Titration Lecture Notes

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Experiment III : Hydrolysis of Butter

Experiment III : Hydrolysis of Butter

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Experiment VII: Determination of Protein Contents by Spectrophotometry

Experiment VII: Determination of Protein Contents by Spectrophotometry

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Experiment IX: Determination of Lipase Enzyme Activity

Experiment IX: Determination of Lipase Enzyme Activity

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Experiment VIII: DNA Isolation And Identification

Experiment VIII: DNA Isolation And Identification

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Polyacrylamide Gel Electrophoresis (Sds-page Method)

Polyacrylamide Gel Electrophoresis (Sds-page Method)

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lab

Practicum 2: Determination Of Acetosal Tablet Contents

Practicum 2: Determination Of Acetosal Tablet Contents

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lab

Practicum 3: Quantitative Analysis Redox Method

Practicum 3: Quantitative Analysis Redox Method

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lab

New Documents from this Course

Urine Chemical Examination

Urine Chemical Examination

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lab

Theoretical Basis Carbohydrate

Theoretical Basis Carbohydrate

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Experiment IV: Determination of Oil Quality

Experiment IV: Determination of Oil Quality

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Experiment III: Analysis Of Proteins

Experiment III: Analysis Of Proteins

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Experiment I: Carbohydrate Analysis

Experiment I: Carbohydrate Analysis

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Experiment V Chemical Hydrolysis of Starch

Experiment V Chemical Hydrolysis of Starch

Buffalo State University CHE 111 | Fundamentals of Chemistry

Study Guide

Theoretical Basis Lipid

Theoretical Basis Lipid

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Determination of Mixture Contents

Determination of Mixture Contents

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Enzyme Experiments

Enzyme Experiments

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Digestion by Pancreasal Sap

Digestion by Pancreasal Sap

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Enzime Digestion

Enzime Digestion

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note

Non-Polar Amino Acid Group Contains S Group

Non-Polar Amino Acid Group Contains S Group

Buffalo State University CHE 111 | Fundamentals of Chemistry

Lecture Note