Osmosis/Diffusion Lab Report Abstract: The effect concentration levels have on the process of osmosis. Intro: Osmosis is the diffusion of water across a semi-permeable membrane. With that being said, diffusion is the random movement of molecules from areas of high concentration to areas of high concentration. The diffusion rate depends on many factors including the size of the molecule. In a hypotonic solution, there is less concentration and more solvent, causing water to enter the cell and make it swell. In a hypertonic solution, there is more concentration and solvent, causing water to leave the cell and make it shrink. In osmosis, water diffuses from a hypotonic solution to a hypertonic solution. The purpose of the experiment is to create a semi-permeable membrane and determine which solution will cause osmosis to occur more effectively. If I submerge the solutions with varying concentrations in water, then the solution with the highest concentration will perform osmosis at optimum rates. Materials and Methods: -dialysis tubes -water -beaker -granulated cylinder -pipettes -sucrose - weighing scale First, I filled five beakers with the same amount of water. Then, I labeled each beaker based on each solutions’ molarity, or the concentration of the solution. The beaker labeled “0 M” was the control variable, so it just had water. Next, in a dish, I weighed amounts of sucrose based on how concentrated each solution was intended to be. Then, I added 20 mL of water to each dish containing sucrose and with a pipette, added my solutions to the dialysis tubes. Then, I tied both ends into small knots and submerged each tube into their respected beakers. After the tubes soaked for fifteen minutes, I weighed the tubes, and recorded my results. Results Molarity Wanted 0.0 M 0.2 M 0.6 M 0.8 M Volume Wanted 20 mL 20 mL 20 mL 20 mL Grams of Sucrose needed 0 1.36 4.104 5.472 1.0 M 20 mL 6.84 Initial Weight Final Weight 0.0 M 0.2 M 0.6 M 0.8 M 1.0 M 0 1.36 4.104 5.472 6.84 0 7.88 11.33 13.65 19.10 % Change in Weight Concentration Change in Weight 0 6.52 7.226 8.178 12.26 % Change in Weight 0 479.41 176.08 149.45 179.2 600 500 400 300 200 100 0 0 0.2 0.6 0.8 1 Molarity Discussion The results from the experiment show that the solution that had a higher concentration level was the heaviest, therefore meaning the liquid level inside the dialysis tube increased as the concentration level increased. As the more concentration increased, the water from inside the beaker moved inside the dialysis tube, causing the weight to increase. The solution with the highest concentration will perform osmosis more effectively because a solution with a high concentration refers to a hypertonic solution. Based on the evidence gathered from the experiment, my hypothesis was verified. There were a few complications in the experimenting process. For instance, when I tied the knots to the dialysis tubes, water would still leak a little which could interfere with accurate results. In addition, when I mixed the solute with the solvent, the solute did not dissolve, so when placed inside the dialysis tube, the solution wasn’t fully used.