University:
Texas A&M University
Course:
BIOL 319 | Integrated Human Anatomy and Physiology I
Academic year:

2023
Views:

412

Pages:

50
Author:

strofamay55

Medical School Histology Basics Blood VIBS 243 lab Objectives are to learn: - the composition and functions of blood - how to identify blood cells - the sizes, distributions, and features/functions of different blood cells -how blood cells are made via hematopoiesis Blood (definition and function) Body Lungs Ref code # 12 Blood - fluid connective tissue composed of erythrocytes (RBC), leukocytes (WBC), and platelets suspended in blood plasma. Function of blood is the transportation of cells and fluid. Function of RBC is to carry O2 to and CO2 from tissues. The cardiovascular system is composed of two sets of closed vessels that encloses blood. Function of blood is transportation of cells and fluid. WBC Immune defense for body. Plasma Nutrients to tissues. Waste from tissues. Proteins to hold water in plasma. Hormones and other informational mediators. Platelets Prevent loss of transportation. Ref code # 14 Ref code #5 Ref code #5 Peripheral blood Unhealthy Blood Smear Image HISTO 021 Reticulocytes Erythrocytes This unhealthy blood smear shows color contrast in reticulocytes before they become erythrocytes Note obvious ribosomes in the reticulocyte; they produce the blue color as seen by light microscopy Reticulocyte Reticulocytes Note ribosomes in the cytoplasm of the reticulocyte are similar in density to that of the cytoplasm of its precursor cell, the orthochromatic normoblast. Both cell types have similar blue cytoplasm under light microschopy Polychromatophilic normoblasts precursor cell to the orthochromatic normoblast Bone marrow 32583 Reticulocyte entering blood vessel Blood vessel Ref code # 5, 12 Eosinophil Neutrophil Lymphocyte Monocyte Basophil Monocyte Ref code # 12 White Blood Cells Neutrophil Eosinophil Neutrophil Eosinophil Basophil Lymphocyte Basophil Monocyte Lymphocyte Monocyte Neutrophil Eosinophil Basophil Lymphocyte Monocyte White blood cells in blood Ref code # 12 Basophil Neutrophil Eosinophil Lymphocyte Monocyte Slide 11 Neutrophils Red blood cells Platelets Large lymphocyte Lymphocyte Monocyte Neutrophil SIZE OF HUMAN BLOOD CELLS Ref code # 14 CELL/PLATELET ERYTHROCYTES LEUKOCYTES (WBC) SIZE 6.5-8 µm % of WBC NEUTROPHIL 12-15 µm 60-70% LYMPHOCYTE 6-18 µm 25% MONOCYTE 12-20 µm 5% EOSINOPHIL 12-15 µm 2-4% BASOPHIL 12-15 µm 0-1% PLATELETS 2-4 µm 110 Peripheral blood smear (Leishman-Giemsa) Neutrophils Monocyte Neutrophils Neutrophils Lymphocyte Monocyte 110 110 Peripheral blood smear (Leishman-Giemsa) Eosinophil basophil, eosinophil, and neutrophils Basophil Neutrophils Neutrophils 110 platelets Eosinophil Neutrophil Red blood cells Eosinophil Neutrophil 113 Peripheral blood smear (May-Grunwald-Giemsa) Monocyte Lymphocyte Monocyte Monocyte Slide 113 Peripheral blood smear (May-Grunwald-Giemsa) Lymphocyte HISTO 021 HISTO 021 60x Eosinophil EM 8a Eosinophil EM 8c Neutrophil Eosinophil Neutrophils and eosinophils in EM 8a Basophil Lymphocyte EM 8c illustrates an example of a basophil Monocyte Basophil 140 Infection site Plasma cells 140 140 Cardiac stomach w/chronic infection Eosinophil Neutrophil Gastric glands EM 12b to observe organelle content differences in an immature and mature neutrophils – mature has more mature dumbbellshaped granules EM 8f EM 8f Lymphocytes EM 6 Note the relative paucity of cytoplasmic organelles and the high nuclear to cytoplasmic ratio (EM 6) Lymphocytes Large Golgi and many plasma membrane projects on monocytes. HISTO 021 Monocytes Large Golgi and many plasma membrane projects on monocytes. 432 Macrophages (Slide 432) lung EM 8f Monocyte HISTO 021 1 Blood monocytes differentiate into macrophages like those seen in the lungs Ref code #5 Hematopoiesis Simplified: Part 1 Erythropoiesis Erythropoiesis Erythrocytes No nucleus and red cytoplasm Reticulocytes No nucleus and blue - grey cytoplasm Orthochromatophilic erythroblasts Dark, small, spherical nucleus blue – grey cytoplasm Polychromatophilic erythroblasts Darkening, fractured ,spherical nucleus and mixed pools of grey and blue cytoplasm Basophilic erythroblasts Fractured, spherical nucleus and thin rim of dark blue cytoplasm Proerythroblasts Uniformly light, spherical nucleus with light nucleoli and thin rim of medium to dark blue cytoplasm Ref code # 6,15 Erythrocytes McGraw-Hill Companies Polychromatophilic erythroblasts Reticulocytes Basophilic erythroblasts Orthochromatophilic erythroblasts Proerythroblasts Erythropoiesis Erythrocytes Proerythroblasts Reticulocytes Orthochromatophilic erythroblasts Basophilic erythroblasts Polychromatophilic erythroblasts Erythropoiesis Bone marrow 32583 Reticulocyte entering blood vessel Blood vessel Bone marrow EM 12a Bone marrow Bone marrow Bone marrow marrow blood EM 8a EM 8b Basophils 432 Lung with bronchi – macrophage Lung with bronchi Ref code #5 Bone marrow Megakaryocyte EM 12c Ref code # 20 The Megakaryocyte has a high density of small vesicles whose membranes will serve as cell membranes when the platelets pinch off from the cytoplasm of the Megakaryocyte .. HISTO 021 Blood Ref code # 2, 12 Blood vessel lumen EM 8f Platelets Blood Megakaryocyte with its cytoplasm extending into the blood stream where the platelets are formed by pinching of parts of the extended cytoplasm.

Medical School Histology Basics Blood

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