Thin Layer Chrmotpgraphy and HPLC

Paper Chromatography →Paper Chromatography in Seperate Aminoacidx a biochemical technique und to paper Chromatography two for the phaser los media seperation of Aminoacids. The phase Stationary ond mobile phase. are ave und are paper chromatography stationary and mobile phater liquids. (The Amino aciels are partitioned bho the liquids. (The what man filter paper in ured as 9 supporting metium Paper Chromatography Principle (The principle involved Can be aphy cor) adsorption Chromatography. partition Chromoteg The and Compounde Hu in the mixture seperate themselves band differences mobik of poves in their affinity toucards stationary phase solvents in the paper. under the Capillary actions Pager Chromatography procedure Selecting It is a Juitable decided bared type of development on the Complexity of the Jolvents paper, mixture usually Ascending type (on) radial paper Chromatography in used to easy to faster form. Also perform handle. The chromatography obteuned and the process is less time Consuming 27 Piepore Sample 2 Selecting a ager in clone paper suitable band Sample quality. filer paper! 00 the filter ves sise of the 3) Prepon the sample: Sample preparation of dissolution in making the He ple in a suitable solvent used Sample Hu mobile phare. > Spot the sample Spotted a at a the paper! Samples should proper position on capillary tube. be paper of using Chromatogram development: Chromatogram clevelopment Spotted tof immersing the paper Due to the Phase moves Capillary over action the Samp Sample on the mobile pheak of paper the mobile the paper. 10 Paper drying & Compound detection: Once the chromatogra is cleveloped Hre paper Also detecting solution developed paper da aried using can be sprayed and dried to Chromatogram spots. air drier, on the chromtogram identify the sample an Paper Chromatography Applications ← 10 Study the process Jermentation and of fermentation ripening To check the purity of Pharma e pharmaceuticals. 10 inspect Cosmetien detect the Contaminanta in drinks and food. Cio examine laboratories. the reaction mixture in biochemical. (To determine dopes and drugs in human and Animals. Typer of paper Chromatography Ascending Chromtography Descending Claromotography Ascending Chromotography Ascending Chromotography the solvents filter paper moves up the Filter paper solverty Tank Descending Chromatography In descending Chromotography Hie folker paper the solvents moves clown Use of paper Chromatography Paper Chromatography in used for the seperation of anino acide from plant tissues Used for Hie saperations of prokins in used tin fou the Identification of Q Amino acids used Amino acicle for the Quantitative Estimation of solvent Thin layer Chromotegraphy (Thin layer Chromotography in to isolate Conducted which A technique used non volatile mixturen. (The experiment in Sheet Coated Aluminium foil, plastic thin with a glass on Prinpiple TLC is based the Rf values Over which on the distribution is the coefficient and support Or matrix Glas plate the thin layer gel in spread which in adiorbed Called the stationary puare mate and mobile phase. Thin layer Chromotography → Thín which layer Chromatography plates roddy mode are Chemically inert and stable. plates are vied -> The stationary phase in applied form of thin layer layer on its surface in the surface The stationary phase on the sige and also has plate has oniform thickness. a tine particle -> TLC to chomber is used to develop plates. Eth responsible keep a Neady envivorment inside which help in developing spots. Also it prevents the solvents evaporation and keeps the entire proces odust TLC mobile phase in the solvent misterve particulate free the one that solvent (This higher movel and consists (This phase should be the quality of purity development of spots in better. Applications of TLC →TLC in extreamly useful in biochemical Analysis such al seperation or from ita bleed iolation of biochemical metabolites plouma, urine body fluids. serum ate, It is widely ix used in formulation seperating multicomponent pharma." uled in thie identify coloure food industry to seperate and used in the Coesmotic Industry used to study if a study if a reaction is complete. TLC chamber Spottin line TLC plate Disadvantages of TLC → The plates do not have Longer Stationary phase. → When compares to others. techniques the Chromotographic length of Seperation in limited. -> The results reproduce. generated from the are difficult to The detection limit is high and therefore if you want a lower detection limit you Cannot USE TLC HPLC (High proformance liquid chromatography) The HPLC is an and effective technique for the reparation quantification of several en their jige or chary. + biological Compounds based It in and analytical chemistry used to quantify each Component in ipporate identify mixture. 0 -> HPLC har been used for manufacturing during the production process of pharmaceutical and bielogread products. → HPLC relien On and adsorbent pumps to pais a pressarized liquid Sample mixture through Components. a column filled with leading to the seperation of the sample → HPLC in distinguished from traditional because operational pressures are significantly highes while evelinary liquid chromatography HPLC Coloumos portides are made with Imaller Adiorbent It is superior to gos chromotography in that it can be unstable and Ured for seperation of thermally non-volatile substance also -> Amino acids, proteine, nucleic acids hydro carbons