Chapter 1: Microbiology Introduction

Chi microbiology introdvetron micro: 10 to the-4 (michumanisms & micrates-SAMETHING) Some are just there and some make you stik -The Jare test? -Inifin bactene is through breastmilk & Vaginal delivery (this becomes part of you then) bacterias aichen Zused to be together / but they were spilt avart fungi Protisis-eukantotes that are single celled. helminths Viruses cand exis on their own-need to harea hest both of these are alive Prons Life on Earth comes from the ocean earliest life forms are prokariotic cells (3.5 billion years ago) mitochandria is passed down from mom (it's maternal) (breastfeeding and vaginal deliving) Endes ymbiotec theory theory that one microbe ate another. Pathogens vs opportuniste. Pathogens Microbes that microbes that cause cause disease disease in a weakened host "true" Pal hogens will ALWAYS catuse a disease -They just hang arove, but if you get sick (your mune systom monken), Diew they attack. They are CoWARAL) je humans Ex-candida allucans groups <1/ofimicnbes actually Petus sick (1400) $alut gramatsitive: can jurrme on skin (can get you very fick if it goes somewhere other than the skin) + Goldentge of michibrology: (1850-1920) Paget 3 need to know what they did in the Golden Age -Robert Hooke (mid 1600'5) thed to describe what cells were Anibre Van Leeuwihbock developed the 1st muroscopes)? first to see bacteria she did share to anyone else. Spontaneous generation vs thogenesis Francesco Rsd Proved that spontaneous generation is true. maggots in meat They didn't believe Louis Pasteur roved that blogemmar is responsible for propagation of lite developed ist raceme against anthrax and rabies proving that pathogens are coming from outside (s-necked flask experiment) - bert koch developed gura theory of disease Koch postulates of disease I same organim mes be presete in every case of diverse 2. Kilate the organism (through Plating) inoculate & prove that microbe is what causes disease (through snowhat of a susceptible bort) ( (re-isolate) 4 trganism must be re-Kolated only 27 of bactena Can be cultured ble its actually reatry hard to grow bactund -1ghar Semmelwris developed the 1st a sephe techn joves Tachlonde solution "Childbed ferer-he recommended handrashing techmane to & mortality rate. - Jomphlister developed extensive Steriletechniques for OR's post-oprotection rate & drastrally for surencal tools -Floveme Nightingale established aseptic techniques in huising mund Care. worked soldiers HAIS-hospital avamined infections (Aktnosocomial intections Scletific method propose hypothesis -collect and analyze okserrations (data) -form a conelusion state whether conclusion supports contradids the hypothesis. law is theary Something that has been tested extenswly. has been disproven, Supports (CCION Closer to fact precise statement/mathematical formula that predicts a specific occurеnсе Taxonomy Strain-different mutation of the same vinas) Pathogen (small differences the have more bacteng in our body that normal cells. Taxanomy-how organisms are gruped by shared features including physical features murta Carl linneus-father of taxommy Domain (largegane) en Koryotic prokaryotes 3 domains Buctena, arches, elukanya found in strange oan fexually annnot reproduce sexually locations extremcophiles deep parts of ocen Turvive extreme Loverritsents Kingdoms plants animals, fungi, Valistic relationship when it organising are closely connected (puresti) pumsite barns the boy mutuation helps the host Consells no beneht of cost to the host. Gut-gram negatives Skin gram positives ATB therapy can kill good bactend cdiff-1x is to stop the ATB that caused it Malang you get it from the tropic you get stekle cell anerete if you hare sickle cell anemia you are less likely to develop maleng -Biofums can form multiple layers and they are very hard to treat capdevelop mteeth, tenses have to be on a living organism) - Agar is addend as a solidifying agent and allows for isolation -most bactena are Fastidious" Bacterial Staining technique Fixation when it sticks to the st the mordant -afre you add the first stain, iodine helps bind (Jodime) locks crystal violet Arrangement of the cells matter in menobiologna) Simple stams Stripural stains (capsule siams-Hs a defense mechamsim) Differential stains Gram Stain & Acid las i storm. विविध ll farget myocello acids between backend to identify 10 121ell Melson' stam. gram(1) purple sangram ( Pink