Fermentation Tests Procedures

University:
Northwest College
Course:
MOLB-2210 | General Microbiology
Academic year:

2023
Views:

201

Pages:

2
Author:

Yesenia M.

Fermentation tests- - Procedures1 Fermentation(broth)- use 1001 to inoculate the fementation tubes with the bacteria- 4-48hrs laterfor growth,acid, gas. VPHincubate the tubes at 35°CisRemember: - examine yellow color fementation (dlt acid production) Remember phenol indicator red!!!red color=< fermentarion.bubble Igas in Durham tube(Fenol Red)ogas= gas = bubblelgas in Durnam tubes2MRVP tests:(Remember - use one tube is MR tube, the other IS tube)a loop and inoculate MRVP test tubes UP WHH bactena- at 35c.- incubate observe for growth, and and gas 24 $48hrs after.24/48hrs later[MR]one tube (mr tube) add 5 drops of methyl red.- (Remember -observe to results (+)result will be red (-) result will acid be pashway (yenov/clear)(1) RESUA means -acid was produced. (Mixed(the mostble #1 its (a) narhthol)V.Preagent\[VP] - -to (Remember and the 0-2ml other reagent (2/3drops) tube (alpha) (VP) 1 is ,alpha add of 9-napthol V-P male 0-6ml reagent I reagent comes 12 DROPS II first) (potassium I of is potassium hydroxide) carefully hydroxize.- cover Think tube a T a parafilm Square and shake tubes (Ifany)willto mix thecads off solution to expose in) media to 02,50 that acetoinCOMMOD Lewsoxidize.allow 15 - 30minutes- - (+) VP test will turn pinkish-red color.(+) VP test means acetoin was produced (neutral products) and PHIST.(-) VP test will remain clear colored.0 aud production. 1 bumatedoil present)3 Citrate Test-use a 1000 ,moculate one utrate slant w/bacteriaYmolulation justa small4- incubate the tubes at 35°Csquiggly is line.look at the tube- If citrate was used ) color will change to blue bk of(t) result = blue color change = citric acid (utiate) was used as thebromythol blue indicator(-) sole result carbon = media source will stay green, 0 citrate was used. /fermented