TEM Analysis - Edubirdie

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

323

Pages:

3
Author:

Sanjeev KG

Principle Same Prinuple as an optical microswpe. In a TEM, electrons replace. photors, electromagnetic lenves replave glass lenses and images are Father than through an eyepiece. Viewed on a sorven It is a microswpr. tochnique. whereby a beam of electro, hansmitted through • an ultra thin specimen, with the specimen as it pass through Interache. An image. B formed from the Interaction of Is thar veriliy Through the spreimen. Emergent & beam & focussed by by objective lene. Final images forme On a flyprescent Suoreen for v •Viewing Component Ane Source →Thermo-lone gun. →Electron beam Electromagnetic lensa. EL → Vacuum chamber. 12 wndenseru. → Sample Stage → Phosphor/fluorescent screen. → Compida/Display. Instrumentation Elechon gun - The is are generated and accelerated Condenser system - Aset-up of different magnetic! and apertures. lens Objective Lens generater - Imp lens in the microscope. since it the fiut Intermediate image. Intermediate diffraction lens - Switching between imaging and mode Projective lensee - furthin magnetration of secund Intermediats Image Image Observation - Image and diffraction pattern Con directly be observed on the viewing soreen. vacciun system - Because of Strong interactions of I with matter, gas particles must be absent in the Lolumn.

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