University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

296

Pages:

2
Author:

Sanjeev KG

Expt. No. 1 Date 3/3/23 Page No. 1 ISOLATION OF MICROORGANISM FROM AIR SAMPLE AIM To isolate microveganism from ail sample and Here by demonstrate crat missorjaniem is ubiquitous in nature. ng PRINCIPLE Miaoorganism are present nearly everywhere in are environment, they ale present in abundantly they are the nubition, moesture of termperature are suitable ta their growth of multiplication. They are in ait we breathe; in food we eat navever it is difficult to prove their presence by direct midoscope obrerwations But sreit presence can be demonstrated by allowing the things such as seal of water to come in contact with the sterile nutrient medioom in misocyanin gau. In this experiment, microbes are allowed to grow on nutrient media that comes from the substance to which the medir are directly exposed. Much call must be faked up to prevent any contamination from other sources. MATERIALS REQUIRED NAM petriplates Heiliged yasswares. PROCEDURE The media was prepared of sterilized. 15-20ml of the media Under aseptic conditionn wae poured into each of the Petriplates The plates were allaved to cool down. Teacher's Signature Expt. No. Date Page No. 2 One plate than nubient ayar was kept open in the environment tor 10 mins while one place was kept as a central All the plates were incubated for 24 to 48 hes at 37°c. The different kind of dones appeared on the plates exposed to the environment was observed of the maphology of the colony were carefully Rudied. RESULTS Alter incubation many idonies were grown in the culture plate The no of colony farming units in the plate which was expered to extrarnival air for 10 mins was Et CFU. The no. of CPUs in the plate without sanitizer was too numerous to count INTC The no. of CFUS in the plate witts sanitiger was GIFUS.