University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

459

Pages:

2
Author:

Sanjeev KG

Expt. No 4 Date 14123 Page No ISOLATION OF RHIZOBIUM FROM A NODULE AIM To wdate thisobeim teom the root nalutes of the leguminous plant PRINCIPLE Rhizobium is a symbidic No fizing organism found in the rod nodules which s legume plante. The agonium is a yas negative aerobic af becomes pleomaphic under adverse conditions the loot nodules weer cleaned a nafare sterilized wing D. 1% MgCl2 4 70% ethand dution boperl the shizobium are isdates of grown media the growth of Rhizobium. on YEMA a specific MATERIALS REQUIRED Tolt-tube, conical Hask, sterilized pipette Platel glass 2.0d spreader, petei- yeast extéaet mannitd agar, eet nodules. neiliged distilled water mercuric chlaide. 70% ethanol, sodium hypochlorit PROCEDURE I wash ecols thoroughly to remove soil. 2. duct about 10 nodules from each plant 3. while moving ins nodule use toe ceps on the root apponda- get to reduce the risk of damaging the nodule. 4. Immerse intact, indamaged nodules 95% ethand to a for sbo 10 cconds in a isopropand 4 then transfer the nodules 2.5-3% solution of sodium hypochlorit sonk Teacher's Signature, td 2-4 min s Rinse in Heill wall of then desiccate the inner Subotanu the nodules & place of the nodules in a sterilined distilled Mix it well with the help of gean eod. 3. Pipette 100ul of sample in the solidified ajar plode al then with the help of spreced plate method + L-shaped glass nod perform the After plating place the plates in the incubator. ind 24 has. at 376 OBSERVATION After 72 hours of incubation isdated small, medium, af large sind orange coloured colonies were observed Upon Geen staining of all is dated organisms. onaped bacteria was observed under the oil on the plates pink daired sod immersion miroscope RESULT The isolated organisms from the nodules rody. (bacillus) are gom negative