University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

335

Pages:

2
Author:

Sanjeev K

iiiINDOLE TESTAIMTo access the ability of the isolate to producetype to pharase enzymeMATERIALS REQUIRED1 Teyptone broth2 Kovac's reagent3. Bacterial culturePROCEDUREandde production is dependent upon the presence oftyptophar in the culture medium1A 1% solution of peptone water is generally used in tefor indole production because it is rich in HyptophBacteria is to be tisled are incubated for 48- 72 ha2.5 drops of Kovac's reagent are added to culture brothIndole reacts with Kovac's reagant to give a cherry3.red ring at the top surface of the test tube. A yecolour indicates a negative resultOBSERVATIONA yellow coloured ring was observed at the tosurface of test tube 16RESULTThe given organism is indde negativeDISCUSSIONIndole lest is abiochemical test which differentialesthe colitian from other members of Enterobacteriacealby relecting their ability to produce the enzymekyptophanase This enzyme hydeolysis the aminoacidtryptophan into indole / pyeuvic acid as NH3 97 is meinteacellular anyyme (endoengyme)