University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

245

Pages:

3
Author:

Amal J.

PRINTenver 1M1H) or UPE.I Core Promotes(notallgenes contain TFIIB recognitionDownstreamallelements)element downstreamPeomoterElementBRETATABRE DInrMTEDPE-25--302-004+DCE+28 to + 32TFIIBInitiatorMotif TendownsrecognitiontreamElementElementElementcoreelementupstream(whichoombeforewith TATAEukpol require Transcription Factors to begin transcriptionTEP (PolI)(BIII)(Pd)TFITFIITFIIID (14)A (2)B (1)F (2) (ajeast-3)factoryE(2)H(10)2 elements for TFIIB recognition - one upstream TATAand 1 downstream IATABREBRE DTATA less promoter contain DPE, TATA and DCE can occur together.II Regulator Peomoter-50to-200 -GCbox & CAAT box TFIID- 14 subunits , first recognises prander1 TBP - TATA box Binding Protein (recognition of+TATA box13 TAF - TBP Associated Factors.TFIID (TBP)- recognise TATA to box) Chelix- major grooverecruite A bendsoA=Tricheaxytomeltbistones not birtoneBlock RNAexit channel.1-mayorstrecruitespecific so bindloB +assymetric bindinddominincit the ONA (open momote2minerminer groovecomplex)transcriptional directionrecruite F + RNApolIIrequite Erequired for intiationrecruite H(x helical motifs)Generally Tf have motifs to bind DNA in the majorgroove Cusually with & helix) , bot TBR with B-pleatedsheat recognise bind a minor gloove and genetate(800)abend then only B can bindB block RNAepcit channelAssymetric binding of Bone domain in mayorgrave (left)one inminor groove (right)transcription proceeds in one directionThis allowbinding ofTFIIB. melt DNA [Block RND exit chanel]- other transcriptionfactorclosedOpen promotecamplesPromoter ATPComplex. TFIIHHelicase activityiKinase activitylargestRNAsuburit-RPB1PolPhosphorylation of 5thserine (kcy event);; P RNA pol can intiate2ndthtranscription)CTD(Hexible)(S)PT(S)P SSeveralrepeatsPrimecandidate of Phosphorylation(human - 50sepeats)Intial movement5thS dephesphorylatedof polymerane in slow5thsafter cappingPPiomoter escapeafter 20-30DockingTFTBleaves (Blocksite of cappingRNDexitchaw)enzymeCap addition after synthesis of 20-30 nucleotideIf capins absent?beforethat they liewhy20-30inside the channel5'- 3'exonodease conrequired - RNAexiteequivecleave mRNAtocap.20-30 nucleotide additionIf RNA5'capersabsentQ(-ve Elongation Factor)factorsNELF,blockblock and inhibit for the elongationfurtherelongal DSIFefthere in no cap/ Pause the psymelaseCURB sensitivity Inducing Factor)CafterkinaseAfter 20 - 30nucleotidesPTTEFBcap2nds Pand addition ofadditionPhotographationPactas docking sitecapNELFDSIFDocking siteremove NELFfor splicing factorsAccelaratedfrom RNAPol.Blockand Polyadenylationtranscriptionremovedtail factors.