University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

62

Pages:

4
Author:

Amal J.

HistorresH1 H2A H2B H3 H4Divaged billionyeaH2A H2BH1Pea and Cowleastmoderatelyconservec(104aminoacids)consaved2 Daminoacid differenceinHuArg - -LygineLeu -esoleurs(1)2H3 2 H4TetramerDNA(H2A), 2(H2A)dimesdoneNNProfi(H3H4).Nfrom2NnodH2AH2B # 2AH2BNNCKaimWatson 222Ntaminus - soseptidto modificationH34 helical domains (extraintubiquitination, methterminsGoner pherphonylation aceteall other - 3.RegulationAcetylation - GeneActivalCHATMethylationActivH2A H2BH2AH2B1330kSAM)stror6030th13bindirH2H3 HHH3 extrasnacextratetramer.NterminesPhosphorylation -terminesthekinase.arObiaritination146bp.APUMwhich histone participate in exit& entry of nudeosome +s N terminal protr utingClocktranscriptionalactivateInacti vationH2A-5,DAcelylation- gone (HAT)H2B-4,8I PhosphorylationH31,113MethylationTorydepends ospositionH4>9,34ObiquitinationPort depends on position5 types of histonemol weightH1beaviest32%H2A20%Moderately 2stDaconselved22%H2BK-RH3 - highly conservedL-V 23%Infiate OctamesH4- Low est mol weightformationhighlyconseived24%Cow Peazamino acid differK-R1L- I2H3+2H4Tetramel - Initiate nucleosomeformation, Interact with middle 60bp of (146)with their 3 x helical domains6/>H4 H3 poces 1 more a helix in Nterminus in teractwith first 13 bp and last 13bp Entryond exit point ofDNA3H3tost2H2H2Bdimer I interact with left 30bp of 606p and2nd interact with All 30bpright to middle 60bp.this3 FCH2A H2BAll these histones poces N terminus tail protrudingoutward from nucleosome chore If histone octamel inmade anotogoos to a clockNNH2AH2B1211H3210NH4N03948N756NN.high myxNis100These N terminus are targets of histone modification metacentricChromosomesubrataraticAcrosentricaTelocentreLonger arm -q arm, shortes arm - palmLargest human ehromosome - IPInterphaseMetaphoxe100mmpackaging size10410ym419100x103Apm 10MM10 heteroctromatinDarkstain, highlycondersed (more stainbes moreong)euchromatinLight stain,chromatin adhere less amountals portion)of staineffectCot CurveCone xtime Curvecradien cresseance(M) (s)mole secondfirst denature and Then senatoreduring repaterationUV.measureCot (MS)absorboneChypoctromk offRevealCotvalue gononic lecomplexity of5y5x106pSimple organism Cotanegenome10 = IC6.x 10x10 bpcomplex genomecomplex patters of Cotarve10% highly. repetitive (satellite)20% moderately repetative CrRNA,transperon (RNA)Cot70% Unique gene (proteincoding)prage (MS) E coli1genefor 1protein50kb4.6x16°6PsimpsoraganismCotanvelong DNAhigh Cotvalue110%1100% DNA as single strondallsingledsDNA20%very0.550% reassociatewickly50%dsDN0% double strand70%0% DNA (100% dsDNrModicate Cotlowestlot