University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

256

Pages:

3
Author:

Amal J.

Eukaryotic TranslationInitiationtRNA bind to rihnome first, after that there will bebinding of mRNAEIF-1, EIIA, RIF-3 , , CIF- 2 (GMPform)elF-5.prevent reassociationCTF-5MGTP14eTF-1eIF-2GTPerr343spreintiationleAC.IF -36eIf- 2 - GTP - facilitate birding of irrtRNmetwith all thepartierto P-siteCBC replaced by aircillary factors in cytoplassFFABInduce helicase activity ofA21capdown streameIFAAG yauxillayETEGA)PAtocap.efactors.42FTGHEG sconnecterG+PABPC interact to circularise the mRNA.eIF4FeTFAA -Atpdependent helicase activity toremove 2° structureCTF4BCIF4G interact with CIF3 to facilitatebinding of 438 preintiation complex with circular mRNA 435+ mRNA485 preintiation complex.1&1 -Ainvolved inGTPscanningeIFHA&B moves5scanningfirst forseanninghelicaseARRY RAMEremove2° structureScanning for start codon by sibosomes, InrtRNAmetinteract with AUG by anticodond Codon interaction, thisbrings a confirmation al change in eIf-5 +(eIfgo from complex) eIF-5 act as GAP - -CGTPase(newconfication)ActivatingPedelrof eIf 2-GTPeIF2-GDP release witheIf5CIF5changecIf5*+ CIF-I leaveGAPleaveseIf-2eFF-2te If-5GTDGDPleavess eIf5B (GTPase) bind to the complexeIf5 - B - GTPin absecen of CIF-I feIf5a 1-ADocking site forlarger subunit.(eIf5 -B - GTP) + FBSeIf 5-B - -GDPleaveswithall other factorAsite freeelongationREFeEFIXGEFe Ef1BrInstead of EFTUinprok)TranslocationeEf2 (instead of EFG ID prok)class