University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

245

Pages:

4
Author:

Amal J.

Genetic Code and TranslationCrenetic codesome connection b/w MRNA andproteintrying to elucidateexpt 1how many possible nucleotides in a genetic ationIf singlet them 4 codon.double 4x4 16 possible codontriplet4x4x4G4 possible codonsGenetic codon is a triplet.2which codon which amino acidEidenburgh and colleguesNeiveroughInvitro translation- 20different testtobes.- All the necessary materials. addedphesphorylase- homopolymens synthesized thing polynudeotideThey are nottenscritied with DNA tempete(1) homopolymer of Uracilradio20amino acid in differenttureand labelled 1 in each tubeIncubationradioactivity.UUUPhesCracked first genetic code UUUPhe mt.UCAGUOUUCUUUC}Phe FUAUUGOUUCCTyr yUACUGC feyscCStop-UAA,UUUAUCA Ser SUAAheu LUGA stopAstop[mt TrpUAG, AGA, AGGUUGUCGUAGUGG Txp wGCOOCCUCAO } His HCG UUERNA32nuclearCUC Lev LCCC Pro PCACCGCCArgR22notCCUACCACGAACAA } Gln QCOGCCGCAGCGGGA00ACUAAC AAU }Asn N.AGU } Ser SUAUCtheu 1AGCCACC[mtmet]Thr TAAAUAACAAAA Lys KAGAAvg R3}AUG Met MACGAAGAGGmstopGCOGAU ZAP DGGUUGUUGACJGGCswittlyGCCCGGUC valAla 7GCAGly GSerAGUAGAAGCGfawGGAGGUGGAGGGG conduct withAAAGGG.2°, structureCCCX for mation.Pro eys tendencyquadruplex stroc (orGGGGboogsteinbasepairingcopolymer mRNA.- repeated sameexpt.radioactivityCA - 2x2x2 8 possible codons.ccc AAA CAA CCA ACC AAC CAC ACA.They couldnt tell which codon codes forwhich amino acid.each codon, got a complex of tRNA, codor. ,aminoacidsynthesiged codons instead of mRNA each tobe64genetic codoo cracked20amino acidsingle amino acid many codons. codos. - degeneray.of genetic genetic codon - -synangmousMet - AUGtrp- UGGonly singlicodon Ssoacceptind tRNAdifferent tRNA which con accept differentcodons of the same amino acid (different ERMAsynonymonscodons),codonanticodon5UUA33'AAUIsoacceptip HRMA5' UUG "3' AAC 5'lev-GtRNA?Degenerate tRNAsingle tRNAdifferent codons ofsame amino acid