University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

325

Pages:

3
Author:

Sanjeev KG

Nuclear Magnetic Spectroscopy fallicu Introduction NMR-A technique & for determining the magnetic moments of nucler by subjecting a substance to high- frequeny radiation and a large magnetic field. NMR Nuclear - Propodies of atomic nuclei-/ Magnetic - Magnetis field is required Resonance Interaction b/w magnetic field and radio frequents Introduction Powerful analytical technique used to characterize, orgaric molecules by identifying Carbon-hydrogen frameworks within molecules. Two typu H-NMR 13C-NMP. The Source Wavelengths, of energy in NMR is radio waves and thue low energy and ficquency. which have long long This waves can H and 13c. change the nuclear spins of sume element, including Frequency in the range of 300 Mhz -500 Mhz. Birth of NMR Principle of Magnetic Resonance. When moleculeس core placed In a • Strong magnetic field, the magnets moments of the nucler align Be Ther equilibrius alignment can State by applying with the field. Can be changed radio frequenry (RF) pulice to an excited 1 • When the nuclei rovert to the equilibrius, they emit RF radiation that can be cletected A spinning Proton Craatse a mag fyld With no external mag field In a magreb The nuclear maanets ame imagnets randomly oriented The nuclear Moar are oriented with • or agamit B NMR Phenomenon +112(01) (or 3) Spinning charge. generala a magnete peld Spin crergy Stat u In the picreme of on antanal magati field (Bo), two two spin state (xst, +1/2 and -1/2