University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

444

Pages:

3
Author:

Sanjeev K

-Role of ATP in Metabolism (masutres) Many rcane completin. in cell are, endorgonuc and will not proceed toward ATP drives such reaction more to completion. ATP-high energy molecule ATP+H₂O ADP+Pi 0 AG = -7.3kcal/mol. A1P has a high Phosphate gp transfer potential. le, it readily transfor its phosphate to wala. So ATP is ideally suited for its role as an energy wrrency. processes such as It is formed in energy trapping and generating photosynthesis, fermentation and asnobic respiraction. Velouk. Vmor Hyberbolic Conve Vinax 2 V= Vmaxt S Km Substrate cons km = the Substrate conc required by the enzyme to operate ad hay its max velocity. Vmax = the rate of poll formation when the enzyme. is saturated wih substrate and operating as fast as possible. Mikhachs Menten Kinetics - The depedance of enzyme activity upon Substrate conc. This Substrate wone fils the MM agn, which relata • velocity (v) to the substrati reaction velou and the Michaeles constant (km).. wnc. (s) using the maximum velocity velo Enzyme, activity vooier 1 Substrate wnc. greally with changes. in environmental fath - wually low within cells. Substrate con core find very low substrate wra, an Nony erreytme makes pat slowly brow il seldom contacts a substrate Molecule. If more substrate molecule cove present, on anzyme bind, Gibstrate more oflen, and the rean velocit • velarty (usually expressed in th of pett formadin) is greater. la than at a lowes substrate con Eg the rate Rate of an enzyme Catalysed ran horcaro with substrate core. Feither I in substrate conc do not result ing a greater Velocity became the available enzyme, molecules are binding Subitos and converting it to polt as rapidly as possible. is, enzyme is Saturated with substrate and maximal vdouty (Vmax): operating al Resulting Substrate conc worne is hyperbola bong Michaelis instant (km) the Subshal for the enzyme to achieve haly maximal veloury wns required 14 is wed as a measure. affinity of an enzyme for Its substrate. of the appoount Clower the kom value, lower the substrate wne at Which an enzyme catalyze its rean.