University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

371

Pages:

3
Author:

Sanjeev K

Continous Cultwe bshata Produl Adden of substrate Ps ortonded to manton the productivity, Chemostat - Limiting nutrient (essential for the growth of organism) we keep on adding the freebmedium the max ul-will be reashed in the Culture vessel so we need to remos the car vol from Custors vessed fur.ex. (IL per how given →IL will be taken out) The alle will be washed out there will not be enough cells fur multiplication. → Dilution rate is very Imp here D= f/v. flow rate Volume. Turbidostad - Predetermined babidity. Continune Culture of Microorganism Substrall Continuer yeter organism grask Growth in an Open system. - Continual provision of nutriend. - Continual removal of waste pdt additional nutriend. Maintaine cells in for extended Periode log phase at a constant biomass Concentration, The Chemostat Rate of Incoming Vessel. medium rate of removal of mediums from An essential nutrient is in limiting quantitia. Dilution rate and Microbial growth.. Cell density/Biomas Measurement Value Guneration time Piluction rate Dilution rate Rate at whch medium flous through Vessel relative to versel size. Cell deneity remained unchanged over a wide range of dilution veder. Chemostat Operates best at low dilution rate The Turbidostat Regulatee the flow rate of media a predetermined tvorbidity/ cell density through veesel to mainten frethimed Ay suppy As filt Culhuc + Use a Photocell to measure the absorbance or turbidity. Dilution reite voories. No Umiting nutrient (all all nutrients are present in excus) furbidostat openides best at high Dilution rates