University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

250

Pages:

3
Author:

Sanjeev K

23/2/23 Microbial Nutrition. Nutrients - Substances wind for energy production and synthesise the Cellular constituents This leads to the growth of organisins. 95% of Microbial cell is dry waight •je, made up of climert. Elements Like Carbon Oxygen Hydrogin. Nitrogen - Presand in Carbohydrate, Protein, lipid and Nucleic acid. Sulphur Phosphorous Potassium kt, essential for enzyme activity. Ca at Prenent in Indospover (Dipicoliner acid) Calcium Mg2+ Mgat Cofactor, Integrity of Cell membrane. Fe. Feat, Fest, Part of cytochrome, è carrying proteins. Crist of Cation's Mgot complexes with ATD stabilise the ribosome and cell membrane Pimciple of Gram Staining. Gram Positive bacteria Teichox and and Lipotector oud heat roristance (Made of Phosphate cont Rubitor) Attached to Myst Alladud to Cea integrity Thek Peptidoglycan Present (40 layers). Permplasmic space (enzymes Present) lell membrane Gam-ve bacteria. Porins. outer membrane. (LPS) Periplasmic Space.. Thin Peptidoglycan. Periplasmic Space Ylell membrane leggered Gram Staining 1) Crystal Violet Gran's lodine iii) Decolorizer (95%. alcohol) 14) Safranine - Counter stan. → First Grystal violet is added and both the gram tue ard-ve are stained. Repeated ont andro Then Gram's lodine is added and H will bind to taichon auch It fixer the stain firmly. But teichon auids are not present in gram-ve nam-ve so it will washed off Decoloverizer is added. There is a rule Called thumbrule as lipids are Soluble in alcohol. Gram tue has NAM and NAGG no lipke. So the porer will Shrink and the decolovicur dewlovarize the background exupt the cell. gram -ve it move into porer and decolonizer it. In gram Laut Counter, Stais is added so the gram tue gram the already hou the colour of 1° stairs so no colaor change but grass negative has no colour so it will take up the colour of the counterstain and appeare as pink. Other alements needed (Trace elements) - Required in a small quantity. Manganese Copper Whey ubiquitous and dor o not usually limit growth. Mistarubiend * Zinc Cobalt part of the enzymes and cofaictor. and aid in the Codrilysis reaction. 3 Molybdenum. -Maintain the Protein Structure. Nickel 7 Zinc a+ Zn Present in the active site -site of the enzyme. It is associated with regi regulatory as well as catalyte Subunits.. Og. Aspartate Carbomys transferase. (E-coli active site) *Manganese Manganese - Mnet Essential for the hansfer of Phosphate gp. (ie, Group transfer, Reaction)./ Cobalt - Part of Vit B12 (cobalamine). Molybdenum - Essential for N₂ fixation.