University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

118

Pages:

3
Author:

Sanjeev K

SEM Brest on scattered as Scatterd is in SEM are Classified as backscattod or 2º electrons - The Scattered as in SEM Produce the Images of the sample after the microscope collects and Count the Scattered Es SEM focuses on the Samples Suofarve and its composition ere 2 million as a SEM only offer max level of magnifiation and a resolution of 0.4mm -Theuko samples in addn, sample Is Hauned by on dement that loption the scattered es- SEM Shows the Sample bit by bit bavongphage Intonal TEM Sud Band on hansmitted as. No other classification of es in TEM Es are directly Pointed towred the sample TEM seeks to see whoet & inside/ beyond the scofan. TEM has uptoa 50 million magnification level and a resoluction of as angstooms. TEM Sample is ut -thirmer. TEM shows the sampleas a whol SEM Provides a 3D image TEM deliver a 2D Picture. Carl Zeiss - Make Optiral len. SEM Instrumentation. + High magnification TEM then only SEM. In SEM and TEM the detector Pont is differens Pe, SEM- Suorfora Analysis, reflection Pattern. up and down So diff TEM-Negative staining. →Structural organisation in microbes (fungi, bautoria, Vion) -TEM fungi and bacteria 10,000 x. Virus-21 magruftation. In light fied 1500 nm. In TEM X~0.2nm. (As low light is used, ma low wavelength). SEM - Analysed by moving the object - we wwe 0-2 ure withrathin specimen. -It can be oor stained / not and Placed on crip and rip ploved in microswliy. TENG-27, black and white, 50 million, good resolution. SEM - Light Sourie tungsten plament lam. - (es are used that is emetted from hingsten flament) In TEM-electromagnetic lens.. - It should be tightly packed as I come wied. We use anode (tue) that help us to the & to pars on both d=A NA distance & magnifxation 1.