University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

178

Pages:

4
Author:

Sanjeev K

Typer of Minisige • Binocular Stereoscopk mikroscope. Scanning probe, mluroscope (spm) •Total Internal reflection fluorescena microscope • Bright field microscope. • Laser microscope (laser scanning onfocal microscope). • Polarizing microscope. Phove Contrast • Multiphoton excitation 1, • Differential Interforanie, untrast, • Shuctured Illumination, • fluorescenu TEM SEM Phase onthart 1 Visualize unstained. Celle. Most of Living Celle. the Staury/ Staining procedures wih kill the cells. It enable •Visualization of living cell.. Working Prinupe The Phave contrast microscopy is based on the Prineple that Small phase changes is the light says, 'induced by the differences in the thicknees and refractive, index of different ports of an object can be transformed into difference in brightness Light intensitice. →A Phase. untiest microswpe, differs from the normal microscope in having two additional components. Sub-Stage. Annular diaphragm. Phase plate Fortioncord cd Phone Plate devard of light retomiling →When the light Passer through an unstained cell, it encounters regions portion iis the cell with different refractive indices and thicknes. -The is difference in the phase blw retarded and an unretarded light rays is about 1/yth of the onginal Wavelength phase hry.. B ouilar len -stge/sample wndensor/dingyingm ligh phouse liyin mat The Phase contrast microscope has Speral darkes what convert there minute changes to amplitude Changes or brightnes changes. → In Phase ontrast misscope. to get loster the diffracted wave, have to separated from the direet waves The separation is achieved by sub-Stage Annular diaphragm.. -The annular diaphragm illuminates the specimen with a hollow cone. of light- → Some rays pass through the thinner ragan of the Specimen and do not undergo any retardation. 1 The rays passing through the denser region of the Specimen get retarded. + The Phare Plate is the retoorded Positioned so that Lighd rays will pass through the area of the phase place and where. light retarding material are coated. When the reborded and unretarded negative Light are recombined or destructive, interference is created, and the Crests and troughs cancel each other. On the other hand if undeviated, light rays core passed through the phase regoording material the two rays will be In the same phare and result in positive / constructive interferone A combination of constructive or destructive interference creates high Applications contrast. in the final Image Vithaled ligh Dilliadid