University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

329

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NO: 187 DATE-10/1/23 BACTERIOPHAGE ISOLATION AM To Molate coliphages team seune complet by plaques assay PROCEDURE a) obtaining bacteriophage benceally bacteriophage con be Jound in places where numerous hosts exist - Because oliphages E.coli as a host oyanism, these phages can be found in places wheel E-coli all plentiful, Sewage colonies contains E. coli remecous sp. coliphage. suwaje also contairs 2) Amdation of bacteriophage Day! Inoculate smi bayphic say beats with E-coli muitate vernight at 37°c. Day 2 moulate mother tale with 4.5ml sewaje, o 5ml of Overnighe E-coli uultule, as 0.5mL of 10x tryptic soy noculate 24-48 hes at 37°C Day 3 1) lentifuge at 2500rpm the labe in the talle top untrifuge for lomis 2) Jake up smt of the supernatant into a see syringe 3) Attach a Ona mivon fille to the base of springe y sille the repernatant in a stecite ture 4) Jable dhe felleate "ensished phage peep" of stole in the repiglotd Pappee notte sample overnight calling & de my 4 Mansfu 100 minclikes of m overnight & code callart inte be antee y tomm peltiplate containing toptic say yus wils a late bent geak nodpread Me pecdetia over the Next place 10 miccolitres of enciched phase prep in the unter & place neebate at 34°a oveenigne Day 5 Cramine your plates the phage magh. ject of kill the bacteria in ue center ne plate, racing a visible region of no bacteria - a plaque obserwalcón A single plaque was observed at the center of the plate A visible region Plate whece was observed at the center of the no bacteria is present. RESULT the bacteriophage successfully killed the huming a plaque bactena DISCUSSION A coliphage is a type of bacteris phage that infects dijam baderia such as E. coli, coliphje ciginate almost exclusively tran numar faces & seom other warm blooded animals they undergo limited replication in sewage & contaminated waters under specific wnditions. such as a high density of susceptible hosts at optimal temperatures All bacteriophaje genome which is enclosed inside encoded capsid proteins. consist of a nucleic acid a shell of phage -