University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

225

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NO: 16 DATE: 20/12/22 ANTIBIOTIC SUSCEPTIBILITY TEST AIM To detect the senselivity of the given borterial illul against antibiotics. PROCEDURE 1 Prepare Muller aganim on -Hinton agar plates finoculate the test the platel by suab cueltas technique. 2. reptically plate different antibiotic dises on the surfall of the plates. 3. huubate the plates at 37°c for 24 heb. 4 Observe the plates for the around each antibiotic disc inhibition zone of each disc presence of inhibition Jones. of measure the size of OBIERVATION RESULT Feon the observation it is cleal that the given agariom was sensitive towards Amoxicillin (Anic), Ofloxacin (OF) Trimethoprion (TR) af infocamphenicol as sones of inhibition was olxeeved disc aland these antibiodic The given cefanism was eesistant to oxacillin af Bacitracin as no sone of inhibition was obeeved around them DISCUSSION Susceptibility is briceria cy singe A lum used when mirobe such a ace unable to grow in the presence of isf more antimicrobial deys Insceptibility testing is performed bideeix or fing causing on naivswal's impechen the way have been secovered in a citrull of the Testing 14 used to determine espravenes of pecepe antithetus to determene of the badwa to stain rate brotes have the portential on the bordersa 4 developed supstance the results of uns can he wed to help select the deuys that well likely. espoliive in beating. 61 injection no most Amoxiullin (ANC) - cared by vaderia used to beat such as ustain injections preumonia, bronchetes Infection of ere ears, mose, Meoat theptococus Enteccions, Haemophilus Amoxicillin ase sesistant to it Nelicobacter Вами subtiles are susceptible t whereas Klebrella & Preudomonas aeruginosa (2) Ofloxacin lungs, prostate und to treat bacterial infections of the skin. a urinaly bact , & aureus spremonia, I pyogenes etc are organisms susceptible to oflaxacin (3) Trimethoprim. It is antibiotic used mainly bladder infections E-coli influenzae, Klebsiella pnevmonia Trimethoprim in the treatment s. pneumoniae, Haemophiles are susceptible to (4) Gracullen used to beat penullin . resestant like S. aureus. (5) asramphenicol - It is antibiotic used for the treatment of no bolecial injections. سوره : It is susuptible to & voli. У вероюсcus pneumoniae. (6) Bautraun. 91 is a polypeptide antibiotic Thex peptides chshupt beam the harderia by interfering with cell wall f Peptidoglycan syneresis Organisms like Saulues. Staphylococcus epidermis 4 Streptococcus pyogenes are susceptib to Bacitracin.