University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

359

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NO: 13 DATE 6/12/22 WIDAL TEST AIM To detect the presence of falmonella antigens by the widal Cost (Rapid Seide Test). MATERIALS REQUIRED. Applicator sticks, disposable plastic deoppers, rubber teats, Cost tubes, Repettes. Namal saline disposable gloves for specimen handling, 5% solution of sodium and calcium rypochedite to wipe and disinfect the surfaces, and disposable biosafety bag to collect and dispose of accessories and waste. PROCEDURE cecan the glass slides supplied in the bit properly of wipe it feel of moisture 2. Place one deep of undetuled test secums in each of the 1st 4 cècles (1 tou) of Ideop of positive conteol selun in each q the last of vicles (5-6). 3. Place one deop of antigens 0.4, A(H) / B(H) in cicles 1,215 44 eespectively at Dantiger in cicle 5 f any of the H antigens EH, A(1) of B(x)}in circle 6. 4. Mix the contents of each cicle with seperate applicator. sticks af speeed it to the whole sees of each individuel cink 5 Rock the hide tor i min of observe for agglutination. If agglutination is visible within I min, the test is positive howed to quantitative slide lest on tube lest tot quantitatie estimation of the titel of appropriate antibody. 6. At no ajglutination is observed, the tot is negative. OBSERVATION Agglutination antigens wns was observed when 1. AH added to 25 ul of test sample. BH, O RESULT The test ecsult is positive ,as contains coceesponding antibodies. the patient secum DISCUSSION wridal test is typhoid or a sedogy blood eest that helps detect enereic jever in the body. This lest was ict conducted in 1896 by Georges Ferdinand widal & was named after him. The widal test is an advanced way to check fr Antibodies bacdetia that the body makes against the salmonella that antibodies in causes typhoid fever. It looks for OTH the patient's seeum. detect life threatening ivernesses This lese helps like typticid fever. The main widal test principle is that the partiink artibody is present in the serum, it will react with a specific antiger af show visible clumping on the test caed