University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

254

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NO: 11 DATE 29/11/22 ISOLATION OF FUNDAL COMPONENTS FROM DIFFERENT ENVIRONMENTAL SAMPLES AIM to isolatt hegal components from different envelormental samples PROCEDURE yar 1 Bespace Lost hong al mediom se egapek dok agae medium in seperate conical flocks. Kerilize those media altry with receiplates for somers at 1216 . serially difull the sample up to 10. 4. specad each ditution in the solidified media. 5. neubals the plates for 3 days at room temp. 6 cucce the plates y identify the colonies through LPCB (lactophenol cotton blue) staining. BSERVATION Furyol stories were green goun feem both shinosphere 4 nen. ehizosphere coil at different dilution mo factor. In 10-2 dilution mal no. of colonies well observed with invease in dilution the no. of colonies welt reduced. On staining the turgal idony With LPCB surgal spoles. hyphae, af feuiting stendures were obtained. RELULT Fungal colonies, hyphae truiting bodies at spoles were observed indet 10X 1 40X DISCUSSION Sunyi all the most divern y adaptable youp of living oyaniems. The coconization density af furyal diversity coom be affected by facties such as salinity, pn, temp., altitude af availability of nutrients. Furjal interaction with ecosistem. to play an important soll in the biodiversity survivabilily Peopagation of productivity of plants established Funge have a beneficial association with numerous hoste af have the capability to degrade environmental pollutants. such as hydescarbons af by products Fungi cef fungal products. site extracellular hy deolytic & lignendegading enzymes, if surfactants offer eco-friendly of cost-effective strategies to address polutios, especially with respect to bidemediation of il spitts & polyacomatic hydeocarbons.