University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

147

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NO: 10 DATE: 22/11/22 TRIPLE SUGAR IRONTEST AIM To determine whetres gom ngative belli diling glucose of Inclose succost famentatively of produce "21. MATERIALS REQUIRED 24 he bacterial wiltule TSI you medium, ajar, distilled wales, streits bet tubes, inoculation loop, burner. PEOLE OURE 1. that the needle containing the pull culletre into the medium, ople the butt of the TSI tube, if ther treak the needle back 4 faith along the surface of the start. 2- Incubate at 37°C ja 18-24 hes- 3. f lacion le suceae) is termented, a large amount of acid is produced which curs the phond eed indicator both in boutt of in the last dane organisms genclate zases, which produces bubbles of lifts the butt on remedium 4 st lachess is net kemented, but the small amount of glucose is, but if the dant will be red (alkaline of nactref pir) 5. It has is produced the black colour of ferrous sulphide is seen. OBSERVATION The slant wat eed in colour but the butt turned yellow along with gas production. RESULT Red plant of yellow buth indicates the organis in it able to ferment dextros only f bubble formation indicates the organisms is able to produce gas. PISCUSSION R TS1 Lest is a misobidogical eest roughly mar named for its ability to cest milo ayarism's abelity to feerment suyals f to produce 135. often and in selective identification of enteric indudiy Calmonella af shigella. Besides TSI is a differential medion thal contain lactare, • a malt oom cunt of glucose which is dezbol ferous nelphate of the pit indicale phened ead diferentiall Carbonytate. It is went to enteric based on ability to reduce & of feerment