University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

465

Pages:

3
Author:

Sanjeev KG

EXPERIMENT NO: 9 DATE-22/11/22 NITRATE REDUCTION TEST AIM To check is differentiation between members of Enterobacteriace en ere base of seu anility to produce nitrate udewage engyme MATERIALS REQUIRED Media Nibals Beath with inverted duchang tuke. Reagents: Sulphalinic acid reagent, a napthylamine seagent, zihe dust Otmas: Inoculating loop, buener, deopper. PROCEDURE howlale nittale heats with a heavy growlts of test agarions. wing aseptic technique. 2 heutate at an appropriate temp. ja 24-48 hours. 3. Add one deopperful of sulfarilic acid af I deopperful fa 2-naphthylamine to each broth. •At this point, a colore charge to sed indicates a the niteale seduction lest If you get a red colour, then you con shop at this point. • No colour change indicates the absence of nitrite This can happen either because nitrate www not reduced of because nitealt was reduced to niteile, then nitrere was fuelkes seduced to some one molecule & you Do not get a sed coloul then you must proceed to next step. 4. Add asomall grout of Zn (a toothpick hell) to each broth. In catalyses the seduction of nebate to neteile At this point, a colour change to red indicates a regative ritrate seduction lest because this means that nitzebe mest have present of must have been reduced No dout change mens thet no nelerly was present. thes ne colocis change et enes point is a poscaine result. OBSERVATION The colour of tubes were some after addition of sulfanilic aud naph truylamine and Indust RESULT The given aganiem is netrale negative DISCUSSION Annecchic metabdin sequices on è accepta consu mom 02. mary gan negative bacterin se nitrate os final é acceptor. Nitrate seduction lest is a test thet detelonines the produll ion of an enzyme called nitrate seductase, which results in the reduction of nitrale (NO3). Bacterial species may be sifferentiated on the basis of ereil ability to reduce netrate to nitele a nitrogenous gases. To determine the ability of on algarion to reduce nitrate toniteite Identify the sijprent ways that nitrute be reduced by bacteria.