University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

52

Pages:

3
Author:

Sanjeev KG

EXPERIMENT-R DATE-15/11/22 CARBOHYDRATE FERMENTATION TEST AIM to determine the chillity of eine geven messeyonims to teement the instechydeals of production of egance and eve product & gasyous ind product. MATERIALS EEQUIRED Terttubes. Jest tube strand, carbohydrate { stucese, mannib case galactose) Fopened set (indicator) basal axdivom, Dueshem's bake, PROLE DURE 1) we weighed the oxidation fermentation basel medivas ton 120 ml soulton of transfered into conical Hasks Didelled water added to peepace a solution. 3) After preparing it is hansferred to a different conical Plaske to the disceed carbohydrate can be added 4) is added ig of glucose, succose, mannitol of galaclose into the 4 desprent conical flask 5) Pherred red was added as an indicator. 6) 30 ml schucos of each conical flask is distributed in tatbes each containing 10ml. (2 tubes ja glicose, 3 tubes for funel. Stubes for mannileh 3 tches for galactose) 7) Then we neeled the Phutham's ticke in a in all the tubes inword position 2) All the tubes were kept for sterilization. 9) Allee steriligation & woling of the tubes weyanins well inculated into & whes keeping 4 tubes as conted with the help of inorintion loop. 10) Apes inccertation the tubes are kept for meubation fre Ju hes at 37°c 11) Alles 24 hes of incubation tos acid al gas product". the tubes are examined. OBSERVATION the tubes in which glucose was present me the carbohydrate the colour of tube changed to yellow but the deeston lube was present at the bottom. The tehes in whion succose was present as the carbohyder shell was no wlour cherrye & the dueshem hebe wes et the bottom the tubes in which mannitol was present as the carbohydra there was doul change of dusham tube was heating. A The tubes in which galactose was present as the carbohydea there was no colour charge if the dusham tube was perseri at the bottom RESULT From the above observation ine given organism is able to ferment glucose by producing acid but thell 4 nie gas production. The aganism is able to ferment mannitol by producing aud as well as gas but the organum is not able to ferment sucrose of galactose as the carbohydrate.. DISCUSSION Paclesia can be differentiated based on their abelily to respire (acecbically, anaerobically or facultative) a theis ability to produce ATP via fermentation. The webohydento fermentation test is used to detect whether or not a bactelia. can utilize. , a certain carbohydrate. It destis ton the Presence of acid af les gas produced teom the fermentatio off singtes particular carbohydrate.