University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

282

Pages:

2
Author:

Sanjeev KG

intercellular enzyme iv METHYL RED AND VORES PROSKAVER TESTS (MR-VP) AIM Te detect the formation of mixed acid (Methyl Red) a Aceton (Veger Proskauer). MATERIALS REQUIRED I neceryl red reagent 2. Bacterial cultures 3. Baccitt's reagent (A/B) PROCEDURE & Methyl red test Inoculate ine aganiem Neubate for 48 his at 37°c. Add a sew deops of methyl sed to eginal illeurs tub 4 mix me contents 3. A red colour indicates indicates a positive result. A yellow a a negative resull. OBSERVATION A red colour the tube was observed at the top surface of RESULT 97 indicates the given organism is MR positive. Vogel-Proskauer test 1. noculate the organim Nurbate for 4t hes at 37℃. 2. Add of Baelitt's scagent & to the tube & mir. 3. Add 15 deops of Brecitt's exagent B almir. 4. The unltull should be nuowed to sit fid about 20 mins tor colour development to occur. 5 Aled colour vindicates a positive result A yellow dow indicatzs a negative, sesult. OBSERVATION A yellow dour was observed at the top surface of the tube RESULT The given deganium is VP negative DISCUSSION The MRVP rest is applied for the invention of teernentatio Pathuray, glucose which is achieved by means of microbes to un 2,3- butanediol af mired and teementation Patthways are the major & solls of pathurys involved. The steady red tone on the surface in the shows positive due to mixed and fermentator ar yellow vwlour resulting negative in the medium patruny MR est. The beounish and tore on the top of medius. Shows positive due to pathway of yellow VP test 2,3-butanediol formentation. dour resulting negative in the