Capsule Staining Reference Materials | Swansea University - Edubirdie

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

332

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NOG DATE 18/10/22 CAPSULE STAINING TEOLE DURE rlace a small cop of Nigessen on the slide- 2. using seerede technique, add a looppel of clever to the slide imeacing it with the dye a spreder sline to deag the mixtull Silm olory Allow inton then the ist slide of Hand for 5-7 mins to nie dey do not heat fix) 5 Flood the imear with crystal violet steun by tilting the wide at 45° angle of let the train sun off until it der dries. Examine the slide under microscope for the presence of Encapsulated codes as wound the cells. indicated by a clear Jone OBSERVATION Under 10x microscope clear white zone was visible. agrinst videt dark background. the capriles were On addition of crystal aven more early visible RESULT Thes confpeme that ine Bacteria is encapsulated. DISCUSSION capsule stain is a type of differential stair which uses acidic af basic dyes to storin the background af bacterial cus easily viscialized respectively, so the presence of capsule is The capsuley is synthesized in the cytoplasm of secrated outside the cech Surrounds the bacterium. where et Most capsulatel baclesia have a capsule made up of polypeptide with virulmce a glycoprotein. Capsules are assouated in soveeal micro organisms, including streptococcus pneumonine af Neisseria meningitides, becauses capsules resist phagocytosis In capsule staining proceduce of rinse the smear "we do not heat fie wien water" as heat I wall may rislodge captures from bacteria.

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