Endospore Staining Study Guides - Edubirdie

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

32

Pages:

2
Author:

Sanjeev KG

EXPERIMENTS DATE-18/10/22 ENDOSPORE STAINING a bar quase ne stide & meht a mese way heute 2 aus dery streat for the organum. acquace of Matting papa the blotting pape with incuca secution of cleam for Smins adhry mal dye sten wied as requited on the gear Ride 4 malachite geen star Keeping une paper moist af on ene slide may be contrine of baling water. wagh the slide with Tap water counterstriss 05% saffeanin ha 30 seconds wath with tapwater I blot dey. - Examine 7 endospore ens videt under microscope for the presence OBSERVATION Under 10 X spores objective cons bright green coloreed were visible of under 100x lens the opsies were even mpee levely visi bale RESULT The beight geen coloured spores compams the presence of Endespoles endospaces ancherial coals have developed DISCUSSION Bacteriol endospace nee metabolically inactive, highly sesistant shuctares produced by corie baderen. as a defensive strategy against unfourable environmen. tal conditions The backera con cermaion in this L suspended state clil condition became favourable if they can geminate of retion to their vegetative state Endospores are bright green in colour of vegetative cells. all brownish eed to pink Spaces may be located in the middle of the cell, at the end of the all the all Spars may may of between the end of middle of Space shape may also be of diagnostic use. be spherical or elliptical.

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