Hanging Drop Method | Swansea University - Edubirdie

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

297

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NO:4 DATE-11/10/22 HANGING DROP METHOD AIM To find out the motility of hactetin MATERIALS REQUIRED CAVITY CLASS SLIDE, overstip culture Petroleum jelly, bacterial wire mourlating loop. PROCEDURE I ikke char cavity wide of coveestip. 2 Apply vaseline / que reound the cavily on the slide Ef wwances of coveeslep 3 Place a deop of bacterial wiltues on the center of coveeslip 4 Carepilly keep tre cavity of the thide over the illiae on overship at gentty press the slide of now the bacterial Enllall hongs. 5 Reverse the slide, so that coveeship is on top of observe fiest in 10x Ef later 4.0x. the cainly OBSERVATION Many rad shaped of circular shaped bacteria were observed moving in the slide. RESULT seve motility of rod shaped bacillus of spherical cor wae found under the mivoscope They change position wels respect to one af another. DISCUSSION Hanging deop peeparation is a modified version of the wel mount method Hele a deop of medios containing the ceganisms is plated on a mileoscopic slide, often used in dark illumination to observe the motility of bacteria Motility is the movement ability of bacteria from one direction to another by theil oun some barteria. ale motele af some ale non-motile. The self propulsion of bacteri'n is detected by one of the following muhanium such as flagellar, gliding a wak soew ay benning motions.

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