University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

79

Pages:

2
Author:

Sanjeev KG

EXPERIMENT NO:3 DATE-11/10/22 GIRAM STAINING AIM To delecmeinde eleven garmpolitive ove jemnegatius MATERIALS FERUIRED brystet motet, crear's rodine, otrylatichel 15% catvanin, yass adide, mutatiope. necheone wiene moculating toop PROCEDURE Jake a clear glass slide.. 2 using stecele technique peepate smeal by placing а мор оf on the slide, the transferring culture reperately to the deep of wall with a sterile, wded oop. & Mix of spread organisms by means of intculating loop a circulal motion of the 4 Allow ameres to air dry of then heat six. Grently hood smears I min- with cystal ndet 4 let it stand te 6 bently wash with tap weter 7 bently Hood imeal with the beam's iodine mordant I Lets stand for I min. f. bently with with tap unter. 9. Decdarrige with 75% ethyl alcohol. Do not over decolis add seagent deop by deop until alwhd suns almost deas, chowing only a blue tinge. 10. bently wash with tap wate lainter Hain with saftanin for 45 sec. 12 bently wash with lap water af air dey ay namine under bil immasion objective. OBSERVATION Preple coloured bod shaped colonies were observed. EESULT The given culture was geam positive sods. DISCUSSION Aram staining s a cerimen lechnique used to diffentiat wr large groups of indecin based on the different ell wact constituents the acom stain praedure distinguisher belwees. Gcam positive and bears negative groups by colouling mese vewlet acam positive bacterin strin videt due to peestrice of a thick layer of peptidoglycon in there all. which retains the aystal vieder mest ulls are Alternatively, bearn negative bacteria which is attributed to a thinner peptidoglycan walls stained with stain sed wall which does not retain the aystal videt during the decorousing peouss.