Atomic Spectrum Microscopy in Microbiology

ATOMIC ABSORPTION SPECTROSCOPY (AAS) Atarnic absorption speelegcopy (AAS) is an absorption spectroscopic method that uses the abrception of light by dece alone in a gaseos state to delesmine the quantitative composition of chemical components. It is used to deleemine the vendaltration of metals present in n sample to be analyzed. It is a highly good analytical technique wiin peat sensitivity PRINCIPLE of a solution containing metal salb (y+x-) is aspeated to the Hame farned a vapd that contains Moms of metal may be A laege no. of gruous metal atom remains in the ground Mate of nee capable of absorbing radiant energy of their specific wavelengon. If the light of resonance wavelength is passed through the flame wrtaining the atoms which all analyse the part of light will be absorbed if the ontent of absception will be directly propalional to the no. of ground stats ateme present in Julie M+x M+ x (Saution) (Mist) MX (solid) Dissociation fr MX (aas) M(gas)+ X (gas) Abrception of radiant energy M(gas) Wen a metal atem is changed into gal & light u parsed from the soulces Me as of nom its milled by absorbing the radiction of a preciculal wavelrych by Beck lambeel's law the chlorbone is givin the logaritromic patio of intensity I jovident right to intensity of abrocbing species. A log To No conc. KLNG stoms where in the pane 6 path lengths theough the fome constant related to absorption coefficient INSTRUMENTION Atormiges: Atomization durace dementi controlled can be carried out eithe by name or neat energy is utilized in AAS to corweet metallic to atornic dinociated vapour by carefully At too high temp. anks are fed into a bapples to burner innourment the temp. thauld be for converken of atomic altrow can be lonized. vapour. Fuel af oxidant mixing chamber which passes the caugh A ribbon Home is produced in AAS The sample is aspirated theough the als into the mixing chamber. 2. Hollow cathode lamp: This lamp uses a Cathode as well as Most other types of light sources. How to fil the cylindrical metal with Ar a Ne gas, which contains the desired dement as an anode. The cathode and anode are subjected to a high voltage, which causes the meth gas to ionize. The cathode material is energized during the glow discharge. to cicade the radiation of the treget element af are Propelled toneds the rathode the gar ions Oher rediation tool tel ased cormerly as clectrode less dischaye lamps if Deuterion Menoche anatre horder to distinguish the radiation specifically to each element hom other sodiation sweated by the radiacin Gaulle ins radiation is then paked via a monochromatoe afis covered anм using richee tracting grafting. a priem mono chhamall or Petectol Monochromatod detects the chosen light onto the detector, commonly a photomultiplice aube te right signal Light intensing into which trans formy an slectrical signal proportional to the Amplifiel the amplifier. the processing of electrical signal is amplipid by Read-out device On read-out devices like metees, the output from the digital display detector is appropriately displayed. H has the ability to show absabance at welding wavelengths as well as the abruption spectrum. APPLICATIONS Analysis of Mg af Ca in tap water. Atermiralión vincial analya's as blood og amount ete malion of trace elements in intaninates soil. in beological Herids such caine, keum ek (niconmental malys face elemente malysis in foocle, cosmeticd, hau etc. Aabra cuticals The amount I stalyse and may be prekrat in trace amounts in finne pharmaceuticals ADVAPUTAGES • kary to use • ascates sensitivity • rapid • Efficient atornijation • Even in small montities of the rampic can be analyzed (50ml) Brindes riches in slid analyzed. of residion jum can be DISADVANTAGES fails to detect non metals. sirvellaneous analysis of elements is not possible. Only able to detect to elements. including earth itals. .