Isolation of Bacteria from Phyllosphere

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

169

Pages:

2
Author:

Sanjeev KG

Expt. No 3 Date 13/3/23 Page No L ISOLATION OF BACTERIA FROM PHYLLOSPHERE AIM Te Molace the tacereia feom phyllaphees. PRINCIPLE The phyllosphere represent one of the largest terrestrial habitatd tos microorganism which inflects ces encil hosts with respect to gauti af resistance to abidic af biotic Heures me niceobial that resides on the phyllosphere ate In addition roughly I billion per saj km of global leaf merface Bacteria are considered to be me dominant micobial inhabitants of phyllosphere, although aechal filamentoul Aingi ef yeasts mary be important. these misoves can be found both as epiphytes on plant surface 4 as endophytes with plant bessives. Shese are 3 major microhabitats where milso- ganum cen inhabit depending on the one that most suppat encel growth with replenie to plants. These microhabitatt all aerial plant part of gone of insuence of esot autem of meernal transport system Phyllespheese is a milohabitat on the surface of plants leaf where diverse group of microorganiums Leconize of caref act weil different afspecific ecological function the diversity of microbial composition of pryllesphere includes algae, bilezia Alamentous fengi yeast of in raze cases neamtodes if protagains The majority of epiphytic bacteria ale commensal fome provide specific evaystem services. such as phylacemediation of toxic Follutants af beogeochemical ascling of important elemen MATERIALS REQUIRED LB agar, persiplales lek tubes. Teacher's Signature FROCEDURE cdtect assesh leaf (node) Yansh Men theodously in running top water Dhy the leasts till it loves its meistare din 3 to 4 days Founder the leaves of weigh ly of weighing balone I add them to stelde wake of serially dilute 4 Now spread plats the ditution in LB ajal places of mechals it at 37°C for 2 days Crewe the plates for wing gearuth of its morphdogy. OBIERVATION Nas 24 hocus of inucbation dated small • white claved were observed awaund different types of leaves. xam staining of all me colonies purple claus, nod traped bacter were observed under 100x oil immersion milope RESULT The done's from the phyllosphere are gem porelive bacullens.