Working of SEM Microscopy

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

270

Pages:

2
Author:

Sanjeev K

EXPERIMENT NO: 7 DATE: 112 2022 SCANNING ELECTRON MICROSCOPE -DEMONSTRATION SEM, popularly known. mope is a beans suryaus as scanning lype of creacios maistas maonope that of microorganim that use a emerges to focu The elutrons are emitte d from the speumen and they play an important primary role of dete mg athe morphology and topography while the backsmattered clubcons INSTRUMENTATION & WORKING the speurme mow conta ast beam of electrons moving as and man speumens The OSEM works of the pinuple of applying the interaction y KE to prodine signals on the electrons (SEM uses erited cleations) These elections are 2º electre ne, baurs colle clatrons and diffracted back scattered elections when are used to new crystallized elements and photone They are wed to produce an image Elections source -Electrons are produced under thermal heat of 3 types of a e sowie voltage 1-40KV. There are ten tungsten in filament, ie, tu num hexaboride and field emusten gun. Laritha *Lenses-ge has several condonser levises tmo tmough ugn est It focus the the forms beam of es from tecure column forming a spot called 'spot size) beam Scanning coil-It deflert the beam over the sufare of the specimen * Delulor, display device, power supply and rauum system. Electrons are chased and fixed wang an cluben gun whrin anderales desen the Armenscope Bassing through a series of rever and apaturd creating a foured beampie there then incerails wirn the en a safare the the sample is contioned stage the chamber, the merercise before a vacuums is created by a serce g pumps The e beam, above abous 2 stan wile control ene porttion the the objective lens, le scans altro is the ene sample and proudes information defined area under study. The interaction between a int sample and the elecrtens will prodire a number signali in the fom f 2 or baik seattered cleeno the detectors in detele there signals. ngnale is prodime a Images whren are duplayed on & computer seveen. The SEM thus produs images by Manning the sample with a imgh enagy beam & clatrons whninis fidely unas medicat seilnu, brolog. um cal sienu, forverhic investigations iti.