University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

371

Pages:

2
Author:

Sanjeev K

EXPERIMENT NO:1 DATE: 29.09.2022 PAPER CHROMATOGRAPHY AIM Separation and identification of aminoacids using Paper inromatography technique. PRINCIPLE Paper enromatography is analytical technique, where separation of components of & mixture takes place due to dif between the differential migration on that occurs Cie, between two immisuble phases phas liquias). It is a type of partition inromatography. where stationary phase is paper (eg: What man no.1 filter paper) and mobile phase is where retardation factor; liquid. The separation difference in par 20 of components ccur due to partition co-efficient, which is related retardation fautor. R Distance travelled by the solute Distance travelled by the solvent MATERIALS REQUIRED Whatman no.1 on a solid aid and/ filter phase) pinned support (stick), samples (shown: (3 known amino (unknown), mobile phase, chromatography you, pernil, scale, capillary tube, Ninhydrin aretone (img/mi) mixed PROCEDURE * Whatman no:1 filter paper of a particular dimension is taken * An ongin line is drawn using seale and pencil, 2cm from the bottom end g. the paper. * Four points are marked with equal distances for spotting the four samples. Three known amino acid samples are spotted: arginine, Alanine, and tyrosive, respectively. The fourth amino acid and sample are unknown. * The samples are spotted and it is allowed to dry