University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

422

Pages:

4
Author:

Sanjeev K

NOTEEnzyme ranneeds StructureModule - 4pathwayNo structuredneededEnzyme is a biomolecule.Energy required to initiate a reaction is called activation energy.Ea-ActiationEnzyme -ProteinApoencymeenergy:Coenzyme - NonPntanascoous organis substance ( og: thatcombines with the specific proteinDiffere b/w loen youe and cofactor - based on theNAD-wengybine how it is boundedcofactor - Non Protein ParthappyFunction of enzymeformation of enzyme substrate Complex. and convert it into productEc numberenzyme commission number Difference b/w Hydrolyance and (yourhydrolyticNon hydrolytic charge leavagecleavageRemoval of functional gp.elimination- - Non hydrolytic clangeRomovalMichaelis- - Menter KineticeV= VmaxtsKmt S.Km = 1/2 Vmax.Hkm = Vmax2lower the Km higher the affinity towards that substrateEnzyme InhibitionCompetitiveNon competitiveCompetitive Inhibition- Compete for the active site of an en namecg:- Succinate Succinate FumaratedehydrogenaeInstead of Star succinate malonic acid be combe andbind to enzyme (malonu acid resem blus the structureof reccinate) lot of treatments it is used.Bacteria require folic acid to grow.Substrate is P- aminobenzale which is used for conen-yme folicacidp- aminobenzoate folale synthage folic and(substrate)Suyanamide resumble a with the p-aminobenzoate So competitive(product)Inhibition takes place. So synthesis of folic acid is blocked andbacteria will die.Non competitive Inhibition - Hg binds and change the Shape of an typeSo the substrate cannot bind to the active site due to thechange in conformation. so no pat / less product will be formedModule -5Stage 2 of catabolim - little bit of energy is generated.8 ATP SynthesisedThe energy synthesised will not be enough for mich bceSo in stage 3 more and more ATP is produced.Stage 1 -No energy generated.fig 3- Break down of Carbohydrate fate, protens generate ATP,NADHNow this energy is available for Anabolism. Glycolyth pathwayPentuse phosphate phospathway is also used to break glucoseHistorglycolysic (EMP)Three pathway for gluce breakdownPentire phosphate parthwayEntor dodary (ED) pathway (PPP)